Zhang Junjie, Li Anjian, He Yang, Guo Taozong, Ge Yixuan, Pan Hongbin, Dong Cuilian
Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.
School of Agriculture and Life Sciences, Kunming University, Kunming, China.
Front Vet Sci. 2025 May 7;12:1577126. doi: 10.3389/fvets.2025.1577126. eCollection 2025.
The misuse of antibiotics threatens animal health and food safety and limits the development of livestock and poultry farming. In animal husbandry, probiotics can be used as an alternative to antibiotics because of their environmental protection and high efficiency. (PG) has positive anti-inflammatory effects in mice; however, its role in other animals remains unknown.
The results showed that cell viability with the PG supernatant was highest at a 20-fold dilution ratio. Exposure of IPEC-J2 cells to LPS (1 μg/mL) significantly increased IL-6 and IL-8 concentrations, which triggered an inflammatory response. PG attenuated the LPS-induced inflammatory response by inhibiting the release of pro-inflammatory cytokines (IL-6) and promoting the release of anti-inflammatory cytokines (IL-10). Transcriptome analysis showed that the average number of clean reads in the LPS and LPS-PG groups was 39,311,061 and 38,085,237, respectively. In total, 2,126 differentially expressed genes (DEGs) were identified between the LPS and LPS-PG groups, including 36 up-regulated and 2090 down-regulated genes. Some DEGs, such as IL-6R and NF-кB, are related to inflammation. GO analysis was used to annotate the functions of the DEGs, and the results showed that biological regulation, cellular anatomical entity, and binding were dominant. KEGG enrichment analysis showed that the DEGs were significantly enriched in the PI3K-AKT signaling pathway, protein export pathway, and antigen processing and presentation pathway.
These results indicate that is a promising probiotic for maintaining and improving intestinal health in piglets.
抗生素的滥用威胁动物健康和食品安全，并限制了畜禽养殖业的发展。在畜牧业中，益生菌因其环保且高效，可作为抗生素的替代品。（PG）在小鼠中具有积极的抗炎作用；然而，其在其他动物中的作用仍不清楚。
结果表明，PG 上清液在 20 倍稀释率下细胞活力最高。将 IPEC-J2 细胞暴露于 LPS（1μg/mL）会显著增加 IL-6 和 IL-8 的浓度，从而引发炎症反应。PG 通过抑制促炎细胞因子（IL-6）的释放和促进抗炎细胞因子（IL-10）的释放来减轻 LPS 诱导的炎症反应。转录组分析表明，LPS 组和 LPS-PG 组的平均 clean reads 数分别为 39311061 和 38085237。总共在 LPS 组和 LPS-PG 组之间鉴定出 2126 个差异表达基因（DEG），其中包括 36 个上调基因和 2090 个下调基因。一些 DEG，如 IL-6R 和 NF-кB，与炎症相关。使用 GO 分析对 DEG 的功能进行注释，结果表明生物调控、细胞解剖实体和结合占主导地位。KEGG 富集分析表明，DEG 在 PI3K-AKT 信号通路、蛋白质输出通路和抗原加工与呈递通路中显著富集。
这些结果表明，PG 是一种有前景的益生菌，可用于维持和改善仔猪的肠道健康。
