Gao Xiaoyu, Li Yanan, Hu Yifan, Yang Weixing, Peng Lei, Sheng Jun, Tian Yang, Yao Lu, Zhao Yan
Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming, China.
College of Food Science and Technology, Yunnan Agricultural University, Kunming, China.
Front Nutr. 2025 May 7;12:1573516. doi: 10.3389/fnut.2025.1573516. eCollection 2025.
Mill. (SA) is a food and medicine homology resource of the Li nationality. In Yunnan folk, people often eat SA "for gut." However, no studies have been reported on its treatment in constipation. The present study evaluated the laxative activity of Mill. aqueous extract (SAAE) using loperamide-induced functional constipation (FC) mouse model. The results demonstrated that SAAE is abundant in nucleotides and polysaccharides. The gavage of 300, 600, and 900 mg/kg·bw of SAAE was efficacious in enhancing defecation behavior and the gastrointestinal transit rate of FC mice. Among these doses, 600 mg/kg·bw of SAAE exhibited the best laxative effect. Furthermore, SAAE exerted a significant effect on the colon transcriptome profiles of FC mice, most notably on the neuroactive ligand-receptor interaction and the TNF receptor type 1 (TNFR1) signaling pathway. Among neuroactive ligand-receptor interaction pathway, SAAE significantly affected the levels of 5-hydroxytryptamine, neuropeptide Y, and epinephrine in the serum and colon of FC mice. In addition, SAAE significantly up-regulated the expression of Bcl-XL, an anti-apoptotic protein in the colon. Intriguingly, SAAE also significantly increased the expression of the neuronal markers HuC/D in the colon. This finding suggests that SAAE may alleviate FC by modulating the enteric nervous system. Notably, SAAE alleviated loperamide-induced gut inflammation, damaged gut barrier, and gut microbiota disruption. A significant increase in the relative abundance of Actinobacteriota, Christensenellaceae, Eggerthellaceae, , and were observed in the gut of FC mice treated with SAAE. These microbial taxa are closely associated with phenotypic indicators of FC, and it is hypothesized that they may be key taxa in SAAE regulation of the gut micro-ecosystems and enteric nervous system to alleviate FC. These findings may contribute to the enhancement of the value and the efficient utilization of SA resources, laying a theoretical foundation for the development of laxative-related products.
糯米藤（SA）是黎族的一种药食同源资源。在云南民间，人们常食用糯米藤来“调理肠胃”。然而，尚未有关于其治疗便秘的研究报道。本研究使用洛哌丁胺诱导的功能性便秘（FC）小鼠模型评估了糯米藤水提取物（SAAE）的通便活性。结果表明，SAAE富含核苷酸和多糖。以300、600和900mg/kg·bw的剂量灌胃SAAE可有效增强FC小鼠的排便行为和胃肠转运率。在这些剂量中，600mg/kg·bw的SAAE表现出最佳的通便效果。此外，SAAE对FC小鼠的结肠转录组图谱有显著影响，最显著的是对神经活性配体-受体相互作用和肿瘤坏死因子受体1（TNFR1）信号通路的影响。在神经活性配体-受体相互作用通路中，SAAE显著影响FC小鼠血清和结肠中5-羟色胺、神经肽Y和肾上腺素的水平。此外，SAAE显著上调结肠中抗凋亡蛋白Bcl-XL的表达。有趣的是，SAAE还显著增加了结肠中神经元标志物HuC/D的表达。这一发现表明，SAAE可能通过调节肠道神经系统来缓解FC。值得注意的是，SAAE减轻了洛哌丁胺诱导的肠道炎症、肠道屏障损伤和肠道微生物群紊乱。在用SAAE处理的FC小鼠肠道中，放线菌门、克里斯滕森菌科、埃格特菌科等的相对丰度显著增加。这些微生物分类群与FC的表型指标密切相关，据推测它们可能是SAAE调节肠道微生态系统和肠道神经系统以缓解FC的关键分类群。这些发现可能有助于提高糯米藤资源的价值和有效利用，为泻药相关产品的开发奠定理论基础。
