Liu Te-Hua, Chen Guan-Li, Lin Chih-Hui, Tsai Tsung-Yu, Cheng Meng-Chun
Department of Food Science, Fu Jen Catholic University, New Taipei City 24205, Taiwan.
Department of Life Science, National Taitung University, No. 369, Sec. 2, University Rd, Taitung City 950309, Taiwan.
Food Funct. 2025 Jan 2;16(1):181-194. doi: 10.1039/d4fo02270j.
Obesity and constipation can alter the intestinal microbiota composition, affecting intestinal barrier integrity, immune function, and metabolic processes. Numerous studies have suggested that and could increase short-chain fatty acid (SCFA) production, thus improving the intestinal microbiota composition, mitigating obesity, and protecting the gastrointestinal tract. Therefore, in this study, we aimed to investigate the impact of TWK10 (TWK10) on gut dysbiosis, obesity, and constipation induced by a high-fat diet and loperamide. Over a 5-week experimental period, rats were fed a high-fat diet and subsequently induced with gut dysbiosis and constipation using loperamide. Concurrently, rats were administered different doses of TWK10 or TWK10-fermented soy milk. Following administration of TWK10 or its fermented soy milk, the expression of adipocyte transcription factors such as PPARγ, C/EBPα, and C/EBPβ proteins and adipocyte size were significantly downregulated ( < 0.05). Regarding intestinal motility, compared to the high-fat diet-induced obesity and loperamide-induced constipation group (L), rats receiving TWK10 or its fermented soy milk exhibited regulation of gastrointestinal hormone levels such as gastrin (GT), somatostatin (Sst), calcitonin gene-related peptide (CGRP), and acetylcholinesterase (Ache) in serum. Additionally, there was a notable increase in the intestinal transit ratio, particularly in the 1X TWK10 group, in which it increased by 10.29% ( < 0.05). Furthermore, the consumption of TWK10 or its fermented soy milk significantly increased the number of goblet cells, as well as the thickness of the muscle and mucosal layers in the colon ( < 0.05). Analysis of SCFA content in fecal samples revealed a significant increase in SCFA concentrations, particularly acetic acid, following administration of both TWK10 and its fermented soy milk. Finally, TWK10 was found to modulate the composition of the intestinal microbiota and increase microbial diversity. In conclusion, TWK10 and its fermented soy milk effectively alleviated loperamide-induced constipation in rats fed a high-fat diet. These findings suggest that TWK10 and its fermented soy milk may potentially be functional foods for promoting intestinal health.
肥胖和便秘会改变肠道微生物群的组成，影响肠道屏障完整性、免疫功能和代谢过程。众多研究表明，[此处原文缺失相关物质]和[此处原文缺失相关物质]可增加短链脂肪酸（SCFA）的产生，从而改善肠道微生物群组成、减轻肥胖并保护胃肠道。因此，在本研究中，我们旨在探究[此处原文缺失物质名称]TWK10（TWK10）对高脂饮食和洛哌丁胺诱导的肠道菌群失调、肥胖和便秘的影响。在为期5周的实验期内，给大鼠喂食高脂饮食，随后用洛哌丁胺诱导肠道菌群失调和便秘。同时，给大鼠施用不同剂量的TWK10或TWK10发酵豆浆。施用TWK10或其发酵豆浆后，PPARγ、C/EBPα和C/EBPβ蛋白等脂肪细胞转录因子的表达以及脂肪细胞大小均显著下调（P<0.05）。关于肠道蠕动，与高脂饮食诱导的肥胖和洛哌丁胺诱导的便秘组（L组）相比，接受TWK10或其发酵豆浆的大鼠血清中胃泌素（GT）、生长抑素（Sst）、降钙素基因相关肽（CGRP）和乙酰胆碱酯酶（Ache）等胃肠激素水平得到调节。此外，肠道转运率显著增加，尤其是在1X TWK10组，增加了10.29%（P<0.05）。此外，食用TWK10或其发酵豆浆显著增加了杯状细胞数量以及结肠中肌肉层和黏膜层的厚度（P<0.05）。粪便样本中SCFA含量分析显示，施用TWK10及其发酵豆浆后，SCFA浓度显著增加，尤其是乙酸。最后，发现TWK10可调节肠道微生物群的组成并增加微生物多样性。总之，TWK10及其发酵豆浆有效缓解了高脂饮食喂养大鼠中洛哌丁胺诱导的便秘。这些发现表明，TWK10及其发酵豆浆可能是促进肠道健康的功能性食品。
