Liu Zhimin, Zhu Jiahua, Gong Ruixue, Wen Zhiyuan, Liu Yanwen, Wang Yulong, Li Zengzhi, Huang Bo, Peng Fan
Anhui Provincial Key Laboratory of Biological Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, Anhui Agricultural University, Hefei, China.
Xuanjiapu Ancient Ginkgo Forest Scenic Area Management Center, Taixing, China.
Microbiol Spectr. 2025 Jul;13(7):e0342524. doi: 10.1128/spectrum.03425-24. Epub 2025 May 22.
is a traditional Chinese medicinal fungus renowned for producing a variety of bioactive compounds, including beauvericin (BEA). BEA has garnered significant attention due to its therapeutic potential and associated food safety concerns. In this study, we identified an ATP-binding cassette (ABC) transporter-encoding gene, , located within the BEA synthesis gene cluster of . Disruption of resulted in a substantial decrease in BEA production. RT-qPCR analysis demonstrated that the loss of significantly downregulated the expression of several BEA synthesis-related genes, including pyruvate kinase, branched-chain amino acid aminotransferase, and ketoisovalerate reductase. Beyond its role in BEA biosynthesis, was found to influence hyphal growth, conidiation, conidial germination, and the oxidative stress response in . Additionally, the knockout strain exhibited a reduced ability to penetrate host cuticles, highlighting the gene's role in fungal pathogenicity. These findings offer a comprehensive understanding of the multifaceted roles of the ABC transporter CcT1 in hyphal development, conidiation, BEA biosynthesis, and stress resistance in . Moreover, targeting presents a promising strategy for reducing BEA content through molecular breeding, thereby enhancing the safety and efficacy of as a medicinal agent.
Beauvericin (BEA) is one of the bioactive components in , a significant medicinal fungus with widespread use in Asia and beyond. BEA also possesses mycotoxin properties, with certain cytotoxicity and potential toxicity. However, few studies report the regulation of BEA anabolism. ABC transporters are a superfamily of membrane proteins and have multiple functions such as regulating fungal metabolism. Here, we report an ABC transporter CcT1 involved in BEA synthesis. The disruption of its encoding gene led to a 64.22% reduction in BEA content compared to the wild-type by regulating the expression levels of several BEA synthesis-related genes. It also affected hyphal growth, conidiation, spore germination, penetration, and oxidative stress resistance of the fungus. The findings in this study enrich the understanding of the function of ABC transporter in fungal metabolism and growth and development.
是一种传统的中药真菌，以产生多种生物活性化合物而闻名，包括白僵菌素（BEA）。由于其治疗潜力和相关的食品安全问题，BEA已引起了广泛关注。在本研究中，我们在的BEA合成基因簇中鉴定出一个编码ATP结合盒（ABC）转运蛋白的基因。的破坏导致BEA产量大幅下降。RT-qPCR分析表明，的缺失显著下调了几个与BEA合成相关基因的表达，包括丙酮酸激酶、支链氨基酸转氨酶和酮异戊酸还原酶。除了在BEA生物合成中的作用外，还发现其影响的菌丝生长、产孢、分生孢子萌发和氧化应激反应。此外，敲除菌株穿透宿主角质层的能力降低，突出了该基因在真菌致病性中的作用。这些发现全面了解了ABC转运蛋白CcT1在的菌丝发育、产孢、BEA生物合成和抗逆性中的多方面作用。
白僵菌素（BEA）是的生物活性成分之一，是一种重要的药用真菌，在亚洲及其他地区广泛使用。BEA还具有霉菌毒素特性，具有一定的细胞毒性和潜在毒性。然而，很少有研究报道BEA合成代谢的调控。ABC转运蛋白是一类膜蛋白超家族，具有调节真菌代谢等多种功能。在这里，我们报道了一个参与BEA合成的ABC转运蛋白CcT1。其编码基因的破坏通过调节几个与BEA合成相关基因的表达水平，导致BEA含量比野生型降低了64.22%。它还影响了真菌的菌丝生长、产孢、孢子萌发、穿透和氧化应激抗性。本研究的结果丰富了对ABC转运蛋白在真菌代谢以及生长和发育中功能的理解。
