用于检测奥罗普切病毒的快速可视化逆转录环介导等温扩增检测法。
Fast and visual RT-LAMP assay for detection of oropouche virus.
作者信息
Silva Livia do Carmo, Silva Diego Michel Fernandes Da, Calassa Igor Marques Cesario, De Curcio Juliana Santana, Costa Luiz Henrique Alves, de Sousa Flávia Barreto, Anunciação Carlos Eduardo, Silveira-Lacerda Elisângela De Paula
机构信息
Center for the Study and Research of (Re)Emerging Agents, Federal University of Goiás, Goiânia, Goiás, Brazil.
Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás, Brazil.
出版信息
Eur J Clin Microbiol Infect Dis. 2025 May 22. doi: 10.1007/s10096-025-05174-w.
BACKGROUND
The epidemic potential of Oropouche virus (OROV) highlights the urgent need for rapid and accessible diagnostic methods to improve testing capacity and reduce result turnaround times, especially in tropical regions where OROV prevalence is rising.
OBJECTIVE
To develop and validate a rapid, cost-effective diagnostic assay for OROV detection suitable for use in resource-limited and field settings.
METHODS
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was designed and optimized for OROV detection. The assay's performance was evaluated in terms of sensitivity, specificity, time to result, and ease of visual interpretation based on colorimetric changes.
RESULTS
The RT-LAMP assay produced results within 30 to 35 minutes and enabled straightforward visual detection through a clear color change. Its detection limit was comparable to that of real-time PCR, the current gold standard for molecular diagnosis. The assay's simplicity and minimal equipment requirements make it well-suited for decentralized testing environments.
CONCLUSIONS
This RT-LAMP assay represents a rapid, sensitive, and accessible tool for OROV diagnosis, with significant potential to enhance outbreak response and surveillance efforts in endemic regions.
背景
奥罗普切病毒（OROV）的流行潜力凸显了迫切需要快速且易于获得的诊断方法，以提高检测能力并缩短结果周转时间，特别是在OROV流行率正在上升的热带地区。
目的
开发并验证一种适用于资源有限和现场环境的快速、经济高效的OROV检测诊断方法。
方法
设计并优化了一种用于OROV检测的逆转录环介导等温扩增（RT-LAMP）检测方法。基于比色变化，从灵敏度、特异性、出结果时间和视觉判读简易性等方面评估了该检测方法的性能。
结果
RT-LAMP检测方法在30至35分钟内得出结果，并通过明显的颜色变化实现了直观的视觉检测。其检测限与实时PCR相当，实时PCR是目前分子诊断的金标准。该检测方法的简单性和对设备的最低要求使其非常适合分散式检测环境。
结论
这种RT-LAMP检测方法是一种用于OROV诊断的快速、灵敏且易于使用的工具，在流行地区加强疫情应对和监测工作方面具有巨大潜力。
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