Jin Rong, Zhao Peng, Yan Mengxiao, Liu Ming, Fan Weijuan, Zhang Qiangqiang, Zhu Xiaoya, Wang Jing, Yu Yongchao, Yang Jun, Wang Hongxia, Tang Zhonghou
Xuzhou Sweetpotato Research Center, Xuzhou Institute of Agricultural Sciences, Jiangsu, China.
Key Laboratory of Sweetpotato Biology and Genetic Breeding, Ministry of Agriculture, National Agricultural Experimental Station for Soil Quality, Jiangsu, China.
Transgenic Res. 2025 May 22;34(1):25. doi: 10.1007/s11248-025-00437-w.
Potassium is a vital element in sweetpotato that plays important roles during its growth and development. In this study, potassium transporter IbHAK5, which is homologous to Arabidopsis HAK5, was cloned and overexpressed in sweetpotato. IbHAK5 encoded a protein of 739 amino acids and localized in the plasma membrane. Two IbHAK5-overexpressing transgenic lines with the highest expression level of IbHAK5 were screened for K-deficiency stress tolerant assay. Compared with wild type sweetpotato plants, transgenic plants grew well with higher chlorophyll content, and maintain great higher K contents via decreasing more K effluxes under low potassium ion (- K) stress condition. Additionally, IbHAK5 can help plants improve root morphology and increase endogenous hormone IAA content under both normal condition and - K stress, which may result in the increased root K absorption ability. The results indicated that IbHAK5 play an important role in sweetpotato response to - K stress, as well as support molecular-assisted breeding with the IbHAK5 gene.
钾是甘薯中的一种重要元素，在其生长发育过程中发挥着重要作用。在本研究中，与拟南芥HAK5同源的钾转运体IbHAK5被克隆并在甘薯中过表达。IbHAK5编码一个由739个氨基酸组成的蛋白质，定位于质膜。筛选出两个IbHAK5表达水平最高的过表达转基因株系进行耐低钾胁迫试验。与野生型甘薯植株相比，转基因植株生长良好，叶绿素含量更高，并且在低钾离子（-K）胁迫条件下通过减少更多的钾外流来维持更高的钾含量。此外，IbHAK5可以帮助植物在正常条件和-K胁迫下改善根系形态并增加内源激素IAA含量，这可能导致根系钾吸收能力增强。结果表明，IbHAK5在甘薯对-K胁迫的响应中起重要作用，也为利用IbHAK5基因进行分子辅助育种提供了支持。
