Quintana-Torres Diego, Valle-Cao Alejandra, López-Soto Alejandro, Folgueras Alicia R
Facultad de Medicina, Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, Oviedo, Spain.
Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Methods Mol Biol. 2025;2930:45-53. doi: 10.1007/978-1-0716-4558-1_4.
The immune system serves as a barrier limiting the initiation and progression of new tumors. Among its cellular components, CD8 T cytotoxic cells are integral to the immune response against cancer. These cells can identify and combat tumor cells by releasing lytic granules, thereby driving the adaptive immune response. However, their function can be hindered by several factors such as immunosuppression in the tumor microenvironment, T cell exhaustion, and immunosenescence. Consequently, dissecting these mechanisms is essential to fully comprehend the immune response against cancer cells and can aid in tailoring future antitumor immunotherapies. In this chapter, we outline a straightforward method for isolating CD8 T cells from the mouse spleen. These cells can then be further analyzed or utilized in in vitro functional studies, such as coculture with target tumor cells and assessing their cytotoxic activity and cytokine production profiling.
免疫系统是限制新肿瘤发生和发展的一道屏障。在其细胞成分中，CD8 T细胞毒性细胞是抗癌免疫反应不可或缺的一部分。这些细胞能够通过释放溶细胞颗粒来识别和对抗肿瘤细胞，从而推动适应性免疫反应。然而，它们的功能可能会受到多种因素的阻碍，如肿瘤微环境中的免疫抑制、T细胞耗竭和免疫衰老。因此，剖析这些机制对于全面理解针对癌细胞的免疫反应至关重要，并且有助于定制未来的抗肿瘤免疫疗法。在本章中，我们概述了一种从小鼠脾脏中分离CD8 T细胞的简单方法。然后可以对这些细胞进行进一步分析，或用于体外功能研究，如与靶肿瘤细胞共培养，并评估它们的细胞毒性活性和细胞因子产生谱。
