从患病伴侣动物中分离出的肺炎克雷伯菌复合体显示出基因组多样性、多重耐药性以及超广谱β-内酰胺酶编码基因。

Klebsiella pneumoniae complex isolated from diseased companion animals reveals genomic diversity, multidrug resistance, and extended-spectrum β-lactamase-encoding genes.

作者信息

de Paula Carolina Lechinski, Rissetti Rafaela Mastrangelo, Yamada Amanda Yaeko, Bertani Amanda Maria de Jesus, Sacchi Claudio Tavares, Campos Karoline Rodrigues, Portilho Fábio Vinícius Ramos, Paz Patrik Júnior de Lima, Bello Thaís Spessotto, Arabe Filho Marcelo Fagali, Panegossi Letícia Colin, Reznik Alec Utida, Ribeiro Márcio Garcia, Camargo Carlos Henrique

机构信息

Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Science, R. Dr. Valter Maurício Corrêa, s/n, Rubião Jr, CEP 18618-681, São Paulo State University-UNESP, Botucatu, SP, Brazil.

Instituto Adolfo Lutz, Av. Dr. Arnaldo, 355, 9o andar, CEP 01246-902, Pacaembu, São Paulo, SP, Brazil.

出版信息

J Appl Microbiol. 2025 Jun 2;136(6). doi: 10.1093/jambio/lxaf128.

DOI:10.1093/jambio/lxaf128

PMID:40402822

Abstract

AIMS

To evaluate the antimicrobial susceptibility and clonal relatedness of Klebsiella pneumoniae isolates recovered from diseased companion animals at a Teaching Veterinary Hospital in Brazil.

METHODS AND RESULTS

A collection of 59 K. pneumoniae complex isolates originating from dogs and cats were subjected to antimicrobial susceptibility testing, multiple antimicrobial resistance (MAR) index determination, polymerase chain reaction for carbapenemase-encoding genes, and molecular typing by pulsed-field gel electrophoresis (PFGE). Nineteen isolates were subjected to whole-genome sequencing (WGS). MAR indices ranged from 0 to 0.73, 50 different PFGE restriction profiles were identified. No carbapenemase genes were detected. Seventeen different sequence types were identified among the 19 sequenced strains, and extended-spectrum β-lactamase genes were detected in five of them.

CONCLUSIONS

Klebsiella pneumoniae isolates from companion animals revealed high genetic diversity, both in PFGE and multi-locus sequence typing analysis; conversely, they presented resistance genes commonly found in human clinical isolates.

摘要

目的

评估从巴西一家教学兽医医院患病伴侣动物中分离出的肺炎克雷伯菌菌株的抗菌药敏性和克隆相关性。

方法与结果

对59株源自犬猫的肺炎克雷伯菌复合体菌株进行抗菌药敏试验、多重耐药（MAR）指数测定、碳青霉烯酶编码基因的聚合酶链反应以及脉冲场凝胶电泳（PFGE）分子分型。19株菌株进行了全基因组测序（WGS）。MAR指数范围为0至0.73，鉴定出50种不同的PFGE限制性图谱。未检测到碳青霉烯酶基因。在19株测序菌株中鉴定出17种不同的序列类型，其中5株检测到超广谱β-内酰胺酶基因。