Functional Roles of Gastrin-Releasing Peptide-Producing Neurons in the Suprachiasmatic Nucleus: Insights into Photic Entrainment and Circadian Regulation.

The suprachiasmatic nucleus (SCN) serves as the central circadian clock in mammals, coordinating daily rhythms in both behavior and physiology. In the SCN, gastrin-releasing peptide (GRP)-producing neurons (GRPNs) are predominantly located in the core region, suggesting their possible involvement in photic entrainment. However, the specific contribution of GRPNs to the regulation of circadian rhythms remains poorly understood. This study utilized a Cre-driver mouse line, knock-in (KI) mice, in which Cre recombinase is exclusively expressed in GRPNs, allowing the selective manipulation of SCN GRPNs to investigate their characteristics and functional roles in circadian regulation. All experiments were conducted in adult male mice. Anatomical tracing revealed that SCN GRPNs primarily project to the thalamus and hypothalamus, whereas input mapping demonstrated that SCN GRPNs receive most synaptic inputs from within the SCN. Behavioral analyses revealed that neither GRP deficiency nor ablation of SCN GRPNs significantly affected circadian locomotor activity rhythms or photic entrainment. However, chemogenetic stimulation of the SCN GRPNs is sufficient to induce phase shifts in behavioral rhythms. Additionally, calcium imaging with fiber photometry indicated that SCN GRPNs quickly responded to photic stimulation, with increased neural activity following retinal exposure to white light. These findings suggest that SCN GRPNs play a role in photic entrainment, albeit potentially redundant with other neuronal populations such as vasoactive intestinal peptide-producing neurons.