Omenge Keziah, Viscardo Ottone Carmelo, De Oliveira Cantao Fernando Rodrigo, Santi Simonetta, van Bel Aart Jan Eeuwe, Musetti Rita
Institute for Biosafety in Plant Biotechnology (SB), Julius Kühn-Institut (JKI) - Federal Research Centre for Cultivated Plants, Erwin-Baur-Straße 27, 06484, Quedlinburg, Germany.
Department of Land, Environment, Agriculture and Forestry (TESAF), University of Padova, Viale Dell'Università 16, 35020, Legnaro, PD, Italy.
Sci Rep. 2025 May 22;15(1):17829. doi: 10.1038/s41598-025-01374-8.
The Arabidopsis seor1ko line, which expresses the protein AtSEOR2 free of its bond with AtSEOR1, exhibits a lower phytoplasma titre as compared to wild type plants. In search for mechanism(s) underlying potential SEOR2-mediated defense responses the transcriptome of healthy wild type and Atseor1ko plants was disclosed by RNA sequencing. Comparative transcriptome analysis revealed 1036 differentially expressed genes (DEGs, 893 up- and 143 down-regulated) between the Atseor1ko line and the wild type. Sequence annotation and classification of the up-regulated genes identified "plant-pathogen interaction" among the most enriched clusters. The "plant-pathogen interaction" cluster included genes encoding members of the protein kinase superfamily, actors in calcium/calmodulin signaling transduction and WRKY transcription factors. An interaction network analysis and a host-phytoplasma interaction map demonstrated that AtSEOR2 protein could interact with the calcium-binding proteins CAM2 and TCH3. The latter one also turned out to be an indirect target of the SAP54 phytoplasma effector, which suggests a SEOR2-mediated role of TCH3 in balancing nutrient investments in plant defense and plant growth.
拟南芥seor1ko株系表达的AtSEOR2蛋白不与AtSEOR1结合，与野生型植物相比，其植原体滴度较低。为了寻找潜在的SEOR2介导的防御反应的机制，通过RNA测序揭示了健康野生型和Atseor1ko植物的转录组。比较转录组分析显示，Atseor1ko株系和野生型之间有1036个差异表达基因（DEG，893个上调和143个下调）。上调基因的序列注释和分类确定“植物-病原体相互作用”是最丰富的簇之一。“植物-病原体相互作用”簇包括编码蛋白激酶超家族成员、钙/钙调蛋白信号转导中的作用因子和WRKY转录因子的基因。相互作用网络分析和宿主-植原体相互作用图谱表明，AtSEOR2蛋白可与钙结合蛋白CAM2和TCH3相互作用。后者也是植原体效应子SAP54的间接靶点，这表明TCH3在平衡植物防御和植物生长的养分投入中具有SEOR2介导的作用。
