Abuseada Hamed H M, Abdel Sattar Osama I, Madkour Ahmed W, Taha Ahmed S
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo, 11751, Egypt.
Sci Rep. 2025 May 22;15(1):17787. doi: 10.1038/s41598-025-99718-x.
The combination of atorvastatin (ATO) and aspirin (ASP) is widely prescribed for preventing cardiovascular diseases, particularly in individuals at risk of atherosclerosis and myocardial infarction. This study aims to develop and validate an eco-friendly spectrofluorimetric method using the first-derivative synchronous fluorescence (FDSSF) technique (Δλ = 80 nm) to simultaneously determine ATO and ASP in combined pharmaceutical formulations. A simple, rapid, cost-effective, and interference-free FDSSF method was employed. Ethanol was used as a green solvent, with a scanning rate of 500 nm/min and Δλ of 80 nm. Distinct fluorescence peaks for ATO and ASP were observed at 384 nm and 365 nm, respectively. The method was optimized for parameters influencing fluorescence intensity, ensuring enhanced sensitivity and selectivity. The method demonstrated excellent linearity (0.4-6 μg/ml for ATO, 1-10 μg/ml for ASP) with high sensitivity, as indicated by low LOD (0.03 μg/ml for ATO, 0.342 μg/ml for ASP) and LOQ (0.248 μg/ml for ATO, 0.714 μg/ml for ASP). The method was successfully applied to commercial tablet formulations and synthetic mixtures, yielding results comparable to HPLC, confirming its high accuracy and reproducibility. Environmental Assessment: The method was evaluated for "greenness" (AGREE, Complex MOGAPI, NEMI), "whiteness" (RGB12), and "blueness" (BAGI), achieving high sustainability scores. The results confirmed its minimal environmental impact and eco-friendly nature. The FDSSF method provides a green, accurate, and reliable approach for simultaneously determining ATO and ASP in pharmaceuticals, offering high sensitivity, exceptional selectivity, and environmental sustainability.
阿托伐他汀（ATO）和阿司匹林（ASP）联合用药常用于预防心血管疾病，尤其是对有动脉粥样硬化和心肌梗死风险的个体。本研究旨在开发并验证一种采用一阶导数同步荧光（FDSSF）技术（Δλ = 80 nm）的环保型荧光分光光度法，用于同时测定复方药物制剂中的ATO和ASP。采用了一种简单、快速、经济高效且无干扰的FDSSF方法。乙醇用作绿色溶剂，扫描速率为500 nm/min，Δλ为80 nm。分别在384 nm和365 nm处观察到ATO和ASP的明显荧光峰。对影响荧光强度的参数进行了优化，以确保提高灵敏度和选择性。该方法具有出色的线性（ATO为0.4 - 6 μg/ml，ASP为1 - 10 μg/ml），灵敏度高，低检测限（ATO为0.03 μg/ml，ASP为0.342 μg/ml）和定量限（ATO为0.248 μg/ml，ASP为0.714 μg/ml）表明了这一点。该方法成功应用于市售片剂制剂和合成混合物，所得结果与高效液相色谱法相当，证实了其高准确性和重现性。环境评估：对该方法进行了“绿色度”（AGREE、复杂MOGAPI、NEMI）、“白度”（RGB12）和“蓝度”（BAGI）评估，获得了较高的可持续性分数。结果证实了其对环境的影响极小且具有环保性质。FDSSF方法为同时测定药物中的ATO和ASP提供了一种绿色、准确且可靠的方法，具有高灵敏度、出色的选择性和环境可持续性。
