Yin Lei, Shen Xuehuai, Yin Dongdong, Hou Hongyan, Wang Jieru, Zhao Ruihong, Qi Kezong, Dai Yin, Pan Xiaocheng
Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Sciences, Hefei, Anhui, China.
Anhui Province Key Laboratory of Livestock and Poultry Product Safety, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Sciences, Hefei, Anhui, China.
Front Cell Infect Microbiol. 2025 May 8;15:1544506. doi: 10.3389/fcimb.2025.1544506. eCollection 2025.
is a member of complex and an emerging zoonotic pathogen. As part of the lymphatic system, the spleen plays a pivotal role in destroying invading pathogens. Various microRNAs (miRNAs) are involved in host resistance to pathogens. However, specific miRNAs that act against remain unknown. Therefore, RNA sequencing (RNA-Seq) of the miRNA profile of the chicken spleen was conducted to further clarify the host immune response to infection. Challenge of 7-day-old chicks with strain AHKV-S01 caused severe damage and enlargement of the spleen. In total, 22 differentially expressed (DE) miRNAs (fold change>2, < 0.05) were identified. Functional annotation analysis of the target genes of DE miRNAs found that signaling pathways related to innate immunity, inflammation, and metabolism were significantly enriched. Notably, expression of gga-miR-2954 was significantly upregulated in the infection group as compared to the control group. , gga-miR-2954 directly repressed luciferase reporter gene activity by binding to the 3' untranslated regions of . Overexpression of gga-miR-2954 in HD11 macrophages significantly inhibited expression of , which is involved in activation of several proinflammatory cytokines. induced damage to the spleen by over activation of inflammatory and innate immune responses. The observed changes to the miRNA expression profile of the chicken spleen elucidate host immune responses to infection, providing critical insights for developing novel therapeutic strategies to enhance chicken resistance against this pathogen.
是复合体的成员，也是一种新出现的人畜共患病原体。作为淋巴系统的一部分，脾脏在破坏入侵病原体方面起着关键作用。各种微小RNA（miRNA）参与宿主对病原体的抗性。然而，针对该病原体起作用的特定miRNA仍不清楚。因此，对鸡脾脏的miRNA谱进行RNA测序（RNA-Seq），以进一步阐明宿主对该病原体感染的免疫反应。用AHKV-S01株攻击7日龄雏鸡会导致脾脏严重损伤和肿大。总共鉴定出22个差异表达（DE）的miRNA（倍数变化>2，<0.05）。对DE miRNA的靶基因进行功能注释分析发现，与先天免疫、炎症和代谢相关的信号通路显著富集。值得注意的是，与对照组相比，感染组中gga-miR-2954的表达显著上调。此外，gga-miR-2954通过与该病原体的3'非翻译区结合直接抑制荧光素酶报告基因活性。在HD11巨噬细胞中过表达gga-miR-2954显著抑制了该病原体的表达，该病原体参与多种促炎细胞因子的激活。该病原体通过过度激活炎症和先天免疫反应对脾脏造成损伤。观察到的鸡脾脏miRNA表达谱的变化阐明了宿主对该病原体感染的免疫反应，为开发增强鸡对这种病原体抗性的新治疗策略提供了关键见解。
