Electricwala A, Ling R J, Sutton P M, Griffiths B, Riley P A, Atkinson T
Thromb Haemost. 1985 Apr 22;53(2):200-3.
The fibrinolytic and thrombolytic properties of a tissue plasminogen activator (tPA) purified from the conditioned medium of an established guinea pig keratocyte (GPK) cell line were investigated in in vitro systems and compared with urokinase. Using the fibrin clot lysis assay, GPK activator appears to be similar to human melanoma tPA and not to human urokinase. GPK activator also caused negligible fibrinogen breakdown, when incubated with human plasma at 37 degrees C over 23 hr. Urokinase on the other hand caused significant fibrinogenolysis, under similar conditions. Comparison of the lysis of plasma clots by GPK activator and human urokinase have shown that GPK activator was a much more effective fibrinolytic agent than urokinase, especially at lower concentrations (less than 50 IU/ml). Studies on the thrombolytic effect of GPK activator on the lysis of aged and cross-linked whole human blood clots and plasma clots hanging in artificially circulating human plasma suggest that GPK activator can lyse both these types of clots equally well. The lysis is dose dependent, attaining complete lysis within 3-6 hr with the concentration of GPK activator in the range of 1-5 micrograms/ml plasma. It is concluded that GPK activator has a higher fibrinolytic and thrombolytic activity and lower fibrinogenolytic activity than urokinase.
从已建立的豚鼠角膜细胞（GPK）系的条件培养基中纯化得到的组织型纤溶酶原激活剂（tPA）的纤溶和溶栓特性，在体外系统中进行了研究，并与尿激酶进行了比较。使用纤维蛋白凝块溶解试验，GPK激活剂似乎与人黑色素瘤tPA相似，而与人类尿激酶不同。当在37℃下与人血浆孵育23小时时，GPK激活剂引起的纤维蛋白原降解也可忽略不计。另一方面，在类似条件下，尿激酶引起了显著的纤维蛋白溶解。GPK激活剂和人尿激酶对血浆凝块溶解的比较表明，GPK激活剂是一种比尿激酶更有效的纤溶剂，尤其是在较低浓度（小于50 IU/ml）时。对GPK激活剂对老化和交联的全人血凝块以及悬浮在人工循环人血浆中的血浆凝块溶解的溶栓作用的研究表明，GPK激活剂对这两种类型的凝块都能同样有效地溶解。溶解是剂量依赖性的，在血浆中GPK激活剂浓度为1 - 5微克/毫升的范围内，3 - 6小时内可实现完全溶解。结论是，GPK激活剂比尿激酶具有更高的纤溶和溶栓活性以及更低的纤维蛋白原溶解活性。
