Lv Kehong, Yue Hongxia, Li Chunyan, Chen Shengzhe, Wang Hongli, Zhang Ruohao, Feng Jing, Zhang Hongjie
State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P.R. China.
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, P.R. China.
Angew Chem Int Ed Engl. 2025 Jul 21;64(30):e202507787. doi: 10.1002/anie.202507787. Epub 2025 Jun 1.
The second near-infrared window (NIR-II) has become an attractive optical region for fluorescence imaging. However, due to the complexity in vivo, NIR-II light with various wavelengths for different imaging scenes has not been investigated. Here, we found that 1525 nm light is suitable for high-resolution fluorescence imaging due to low background interference, while its attenuation in vivo makes it unsuitable for deep imaging. The 1064 nm light is suitable for deep imaging. The impact of Yb ions and the proportion of the intermediate layer in lanthanide nanoparticles on the modulation of emissions were investigated. The intense 1064 nm emission is achievable when the Yb content is 80% and the intermediate layer proportion is 0.1. The bioorthogonal orthotopic liver tumor imaging could be achieved by modifying the lanthanide nanoparticles with DBCO-PEG-DSPE and establishing artificial receptors by N-azidoacetylmannosamine-tetraacylated (AcManNAz). Lanthanide nanoparticles with the intermediate layer proportion of 0.66 and 80% Yb facilitate intense 1525 nm emission, enabling the vascular imaging. Based on the imaging characteristics of NIR-II emissions with different wavelengths in vivo, this work achieves high-resolution imaging and deep imaging through intermediate layer regulation of lanthanide nanoparticles, providing an opportunity to explore a new generation of high-performance fluorescent probes.
第二近红外窗口（NIR-II）已成为荧光成像中一个有吸引力的光学区域。然而，由于体内情况复杂，尚未对适用于不同成像场景的各种波长的NIR-II光进行研究。在此，我们发现1525 nm光由于背景干扰低而适用于高分辨率荧光成像，但其在体内的衰减使其不适用于深度成像。1064 nm光适用于深度成像。研究了镧系纳米粒子中Yb离子和中间层比例对发射调制的影响。当Yb含量为80%且中间层比例为0.1时，可实现强烈的1064 nm发射。通过用DBCO-PEG-DSPE修饰镧系纳米粒子并用N-叠氮乙酰甘露糖胺四酰化（AcManNAz）建立人工受体，可实现生物正交原位肝肿瘤成像。中间层比例为0.66且Yb含量为80%的镧系纳米粒子有利于强烈的1525 nm发射，从而实现血管成像。基于体内不同波长NIR-II发射的成像特性，这项工作通过镧系纳米粒子的中间层调控实现了高分辨率成像和深度成像，为探索新一代高性能荧光探针提供了契机。
