Bartuskova Hana, Kralova Lesna Ivana, Kauerova Sona, Lanska Vera, Fronek Jiri, Janousek Libor, Muffova Barbora, Paukner Karel, Poledne Rudolf
Atherosclerosis Research Laboratory, Experimental Medicine Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic.
Adipocyte. 2025 Dec;14(1):2499500. doi: 10.1080/21623945.2025.2499500. Epub 2025 May 23.
Inflammatory changes in perivascular adipose tissue are associated with atherosclerotic lesions in the adjacent artery and can also be used as a marker in patient workup. While adipocyte size is known to be closely related to adipose tissue dysfunction and inflammation, it has not been widely studied in perivascular adipose tissue obtained from healthy human subjects without clinical atherosclerosis. In this cross-sectional study, we addressed this issue by measuring adipocyte size and defining its relationship to cardiovascular risk factors in a healthy cohort of living kidney donors. The presence of cardiovascular risk factors was established by a standardized questionnaire, clinical measurements and body composition analyses. Adipocyte size was measured in the perivascular depot. The proportions of various macrophage subtypes were determined by flow cytometry. To confirm the results, the proportion of CD68 + macrophages was additionally assessed by immunohistochemistry. A correlation and principal component analyses were performed to explore associations. Adipocyte size in perivascular adipose tissue correlated with markers of lipid metabolism, inflammation, and glucose metabolism. Further, the positive correlation with the pro-inflammatory subpopulation of macrophages suggests a strong local effect of perivascular adipose tissue. Perivascular adipocyte size was associated with cardiovascular risk factors and markers of inflammation in a healthy cohort of living kidney donors. This further supports the local role of adipose tissue dysfunction and inflammation in early atherosclerosis development and detection.
血管周围脂肪组织的炎症变化与相邻动脉的动脉粥样硬化病变相关，也可作为患者检查的标志物。虽然已知脂肪细胞大小与脂肪组织功能障碍和炎症密切相关，但在无临床动脉粥样硬化的健康人类受试者的血管周围脂肪组织中尚未得到广泛研究。在这项横断面研究中，我们通过测量脂肪细胞大小并确定其与健康活体肾供体队列中心血管危险因素的关系来解决这个问题。通过标准化问卷、临床测量和身体成分分析确定心血管危险因素的存在。在血管周围脂肪库中测量脂肪细胞大小。通过流式细胞术确定各种巨噬细胞亚型的比例。为了证实结果，通过免疫组织化学额外评估CD68 +巨噬细胞的比例。进行相关性和主成分分析以探索关联。血管周围脂肪组织中的脂肪细胞大小与脂质代谢、炎症和葡萄糖代谢的标志物相关。此外，与促炎亚群巨噬细胞的正相关表明血管周围脂肪组织具有强大的局部作用。在健康活体肾供体队列中，血管周围脂肪细胞大小与心血管危险因素和炎症标志物相关。这进一步支持了脂肪组织功能障碍和炎症在早期动脉粥样硬化发展和检测中的局部作用。
