Puvion-Dutilleul F, Bachellerie J P, Bernadac A, Zalta J P
C R Acad Hebd Seances Acad Sci D. 1977 Feb 21;284(8):663-6.
Miller Beatty's technique was adapted to the study of definite chromatin fractions (nucleolar and nonnucleolar chromatin) isolated from Mammalian cells. The ultrastructural organization of the transcriptional complexes obtained depended on the nuclear compartment studied. In isolated nucleoli, there were "Christmas-tree"-like figures. In nonnucleolar chromatin, the figures were different from the former by the internal structure of the RNP fibrils being synthesized.
米勒·比蒂的技术被应用于对从哺乳动物细胞中分离出的特定染色质组分(核仁染色质和非核仁染色质)的研究。所获得的转录复合体的超微结构组织取决于所研究的核区室。在分离出的核仁中,有“圣诞树”样结构。在非核仁染色质中,这些结构与前者不同,在于正在合成的核糖核蛋白纤维的内部结构。