Morris B J
Clin Chim Acta. 1977 Mar 15;75(3):503-7. doi: 10.1016/0009-8981(77)90374-6.
EDTA, 2,3-dimercaptopropanol and sucrose were shown by Scatchard analysis to interfere in the radioimmunoassay of angiotensin I by altering the affinity, but not the capacity, of antibody for antigen. It is stressed that when interfering reagents are present in samples the assay result must be obtained from a standard curve in which each standard contains the interfering reagent(s) at a similar concentration as present in each sample mixture.
通过Scatchard分析表明，乙二胺四乙酸（EDTA）、2,3-二巯基丙醇和蔗糖会改变抗体对抗原的亲和力，但不影响其容量，从而干扰血管紧张素I的放射免疫测定。需要强调的是，当样品中存在干扰试剂时，测定结果必须从标准曲线中获取，其中每个标准品所含干扰试剂的浓度应与每个样品混合物中的浓度相似。
