肠炎沙门氏菌鼠伤寒血清型利用VI型分泌系统来促进细菌适应性并增强其在肉鸡体内生态位的生存能力。

Salmonella enterica serovar Typhimurium uses T6SS to promote bacterial adaptability and enhance its survival of niches in broiler chickens.

作者信息

Chen Huimin, Shang Ke, Jia Yanyan, Guo Rongxian, Li Jing, Liao Chengshui, Ding Ke, Chen Songbiao

机构信息

College of Animal Science and Technology/ Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang 471023, PR China; Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Henan University of Science and Technology, Luoyang 471003, PR China; The Key Laboratory of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang 471023, PR China.

出版信息

Poult Sci. 2025 May 8;104(8):105277. doi: 10.1016/j.psj.2025.105277.

DOI:10.1016/j.psj.2025.105277

PMID:40412062

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12151195/

Abstract

The type VI secretion system (T6SS) is a specialized protein complex in Gram-negative bacteria that delivers toxic effector molecules into target cells. However, the role of ClpV-a critical ATPase involved in T6SS assembly in Salmonella pathogenesis remains poorly understood. In this study, we investigated the contribution of ClpV to environmental stress resistance and virulence in Salmonella enterica serovar Typhimurium (S. Typhimurium). Our findings demonstrate that ClpV significantly influences the ability of S. Typhimurium strain SL1344 to with stand various stressors, including bile salts, acidic conditions, hydrogen peroxide, and ethanol. Furthermore, ClpV enhances the competitive fitness of S. Typhimurium against commensal gut bacteria. Notably, ClpV appears to play a crucial role in pathogenicity by modulating the gut environment, disrupting microbial homeostasis, and facilitating bacterial persistence in host niches. These results provide a foundation for future studies on the molecular mechanisms by which T6SS mediates gut colonization and chronic infection in S. Typhimurium.

摘要

VI型分泌系统（T6SS）是革兰氏阴性菌中的一种特殊蛋白质复合体，可将有毒效应分子传递到靶细胞中。然而，ClpV（一种参与T6SS组装的关键ATP酶）在沙门氏菌致病机制中的作用仍知之甚少。在本研究中，我们调查了ClpV对鼠伤寒沙门氏菌（S. Typhimurium）环境应激抗性和毒力的贡献。我们的研究结果表明，ClpV显著影响鼠伤寒沙门氏菌SL1344菌株耐受各种应激源的能力，包括胆盐、酸性条件、过氧化氢和乙醇。此外，ClpV增强了鼠伤寒沙门氏菌对共生肠道细菌的竞争适应性。值得注意的是，ClpV似乎通过调节肠道环境、破坏微生物稳态以及促进细菌在宿主生态位中的持续存在，在致病性中发挥关键作用。这些结果为未来研究T6SS介导鼠伤寒沙门氏菌肠道定植和慢性感染的分子机制奠定了基础。