基于新型双特异性单克隆抗体的双分析物免疫层析试纸条用于检测谷物样品中的黄曲霉毒素B1和赭曲霉毒素A。
Novel bispecific monoclonal antibody-based dual-analyte immunochromatographic strip for the detection of aflatoxin B1 and ochratoxin A in cereal samples.
作者信息
Wu Shih-Wei, Tai Wei Hong, Hsieh Chia-Yu, Liu Biing-Hui, Yu Feng-Yih
机构信息
Graduate Institute of Toxicology, College of Medicine, National Taiwan University, No.1, Sec. 1, Jen Ai Rd, Taipei 100, Taiwan.
Department of Biomedical Sciences, Chung Shan Medical University, No.110, Sec. 1, Chien Kuo N. Rd, Taichung 402, Taiwan.
出版信息
Food Chem. 2025 Sep 30;487:144762. doi: 10.1016/j.foodchem.2025.144762. Epub 2025 May 17.
We have developed a novel bispecific monoclonal antibody (mAb) for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA). The specific mAb was used to establish a sensitive competitive direct ELISA (cdELISA) and dual-analyte immunochromatographic strip (immunostrip). The cdELISA achieved IC values of 0.06 ng/mL for AFB1 and 1.02 ng/mL for OTA, with limits of detection (LOD) of 0.004 ng/mL and 0.24 ng/mL, respectively. The dual-analyte immunostrip exhibited visual detection limits of 0.5 ng/mL for AFB1 and 5 ng/mL for OTA. Recovery rates for spiked cereal samples ranged from 91 % to 110 % for AFB1 and from 99 % to 107 % for OTA. The analysis of 15 cereal samples using the cdELISA detected AFB1 and OTA contamination consistent with HPLC analysis, validating the reliability of both methods. This bispecific mAb offers a rapid and efficient approach for monitoring AFB1 and OTA in food, enhancing food safety efforts.
我们开发了一种新型双特异性单克隆抗体（mAb），用于同时检测黄曲霉毒素B1（AFB1）和赭曲霉毒素A（OTA）。使用该特异性单克隆抗体建立了灵敏的竞争性直接酶联免疫吸附测定法（cdELISA）和双分析物免疫层析试纸条（免疫试纸）。cdELISA对AFB1的IC值为0.06 ng/mL，对OTA的IC值为1.02 ng/mL，检测限（LOD）分别为0.004 ng/mL和0.24 ng/mL。双分析物免疫试纸对AFB1的目视检测限为0.5 ng/mL，对OTA的目视检测限为5 ng/mL。加标谷物样品中AFB1的回收率为91%至110%，OTA的回收率为99%至107%。使用cdELISA对15个谷物样品进行分析，检测到的AFB1和OTA污染情况与高效液相色谱分析结果一致，验证了两种方法的可靠性。这种双特异性单克隆抗体为监测食品中的AFB1和OTA提供了一种快速有效的方法，加强了食品安全保障工作。
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