Zhang Yifan, Tan Jiafeng, Tu Xiaoliang, Tan Dengfeng, Yu Lanqin, Xie Yijian, Li Na, Zhang Shuyun, Li Lihua, Zhou Changren
Guangdong Provincial Key Laboratory of Spine and Spinal Cord Reconstruction, The Fifth Affiliated Hospital of Jinan University (Heyuan Shenhe People's Hospital), Jinan University, Heyuan 517000, China; Department of Materials Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China.
Department of Materials Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China.
Biomater Adv. 2025 Nov;176:214350. doi: 10.1016/j.bioadv.2025.214350. Epub 2025 May 19.
Infected chronic wounds resulting from bacterial, viral, or other microbial invasion disrupt the normal wound-healing process, often prolonging recovery and requiring surgical intervention. A high nitric oxide (NO) concentration damages bacterial DNA and disrupts metabolic functions, indicating its effective antibacterial potential, whereas a low NO concentration exerts detectable provascularization activity. These properties render NO an effective treatment agent for infected chronic wounds. However, challenges remain in generating NO efficiently, controlling its dosage, and selecting an appropriate carrier for wound treatment. To overcome these problems, we synthesized S-nitrosoglutathione (GSNO) and thiolated alginate (SA-SH). GSNO was used as the NO source for continuous release. The content of the thiolate group in SA-SH was 158.10 ± 10.84 μmol/g. The GSNO and SA-SH mixture was quickly solidified in the presence of calcium ions to obtain GSNO/SA-SH hydrogel (SSG). This mixture is suitable for treating irregular wounds. In addition, early release of high-dose NO inhibits bacteria (first 72 h), whereas late release of low-dose NO promotes angiogenesis (72-144 h). Experiments conducted on a rat infection model showed that the release of NO reduced infection and effectively inhibited bacterial invasion of tissues. On the 14th day after surgery, the healing rate of the control group was 82 %, whereas that of the SSG group was 100 %. The degree of angiogenesis in the SSG group was 1.5 times that in the SA-SH group, indicating that NO accelerates angiogenesis. Thus, a rapid-forming, NO releasing dressing can effectively treat infected wounds.
由细菌、病毒或其他微生物入侵导致的感染性慢性伤口会破坏正常的伤口愈合过程，常常延长恢复时间并需要手术干预。高浓度的一氧化氮（NO）会破坏细菌DNA并扰乱代谢功能，显示出其有效的抗菌潜力，而低浓度的NO则具有可检测到的促血管生成活性。这些特性使NO成为治疗感染性慢性伤口的有效治疗剂。然而，在高效生成NO、控制其剂量以及选择合适的伤口治疗载体方面仍然存在挑战。为了克服这些问题，我们合成了S-亚硝基谷胱甘肽（GSNO）和硫醇化海藻酸盐（SA-SH）。GSNO用作持续释放的NO源。SA-SH中硫醇基团的含量为158.10±10.84μmol/g。GSNO和SA-SH混合物在钙离子存在下迅速固化，得到GSNO/SA-SH水凝胶（SSG）。这种混合物适用于治疗不规则伤口。此外，高剂量NO的早期释放可抑制细菌（最初72小时），而低剂量NO的后期释放则促进血管生成（72-144小时）。在大鼠感染模型上进行的实验表明，NO的释放减少了感染并有效抑制了细菌对组织的侵袭。手术后第14天，对照组的愈合率为82%，而SSG组的愈合率为100%。SSG组的血管生成程度是SA-SH组的1.5倍，表明NO加速了血管生成。因此，一种快速形成、释放NO的敷料可以有效治疗感染伤口。
