Xu Yawen, Zhang Hailing, Jiao Xuehao, Zhang Yanbo, Yin Ge, Wang Cui, Du Zengkan, Liang Meng, Gao Xin, Gu Zhengsheng, Jiang Yan, Du Bingying, Bi Xiaoying
Department of Neurology, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, PR China.
Department of Neurology, Dalian Municipal Central Hospital Affiliated to Dalian University of Technology, Dalian, PR China.
NPJ Aging. 2025 May 25;11(1):42. doi: 10.1038/s41514-025-00228-x.
Vascular dementia (VaD) is the second most common cause of dementia. Few bioinformatic analysis has been done to explore its biomarkers. This study aimed to excavate potential biomarkers for VaD using bioinformatic analysis and validate them at both animal and patient levels. Based on microarray data of GSE122063, bioinformatic analysis revealed 502 DEGs in the frontal and 674 DEGs in the temporal cortex of VaD patients. Afterward, the hub genes between two regions, including C1QA, C1QB, C1QC, and C5AR1, were dugout. Interestingly, compared with sham mice or controls, the above four complements were highly expressed in the cortices of VaD animals and in the peripheral serum of VaD patients. Moreover, receiver operating characteristic curve analysis conformed to good diagnostic powers of these complements, with C1QB having the most prominent capacity (AUC = 0.799, 95%CI 0.722-0.875). That means the complements, especially subunits of C1Q, might be used as specific early VaD diagnostic biomarkers.
血管性痴呆（VaD）是痴呆的第二大常见病因。目前很少有生物信息学分析用于探索其生物标志物。本研究旨在通过生物信息学分析挖掘VaD的潜在生物标志物，并在动物和患者层面进行验证。基于GSE122063的微阵列数据，生物信息学分析显示VaD患者额叶有502个差异表达基因（DEG），颞叶皮质有674个DEG。随后，挖掘出两个区域之间的枢纽基因，包括C1QA、C1QB、C1QC和C5AR1。有趣的是，与假手术小鼠或对照组相比，上述四种补体在VaD动物的皮质和VaD患者的外周血清中高表达。此外，受试者工作特征曲线分析表明这些补体具有良好的诊断能力，其中C1QB的能力最为突出（AUC = 0.799，95%CI 0.722 - 0.875）。这意味着补体，尤其是C1Q的亚基，可能用作VaD特异性早期诊断生物标志物。
