Songdech Pattanan, Jayasekara L A Channa Bhathiya, Watchaputi Kwanrutai, Butkinaree Chutikarn, Yingchutrakul Yodying, Soontorngun Nitnipa
Excellent Research Laboratory for Yeast Innovation, Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Luang, Pathum Thani, 12120, Thailand.
Sci Rep. 2025 May 25;15(1):18233. doi: 10.1038/s41598-025-03061-0.
Biosurfactants offer good advantages over synthetic counterparts, including biodegradability, environmentally friendly and low toxicity. This study employed a yeast Meyerozyma guilliermondii MX strain for bioconversion of lignocellulosic xylose and palm oil to valuable glycolipid biosurfactant with desirable properties. The objective was to elucidate metabolic pathways related to production of glycolipids and its functional properties. To enhance de novo glycolipid production, manipulation of responsible enzymatic genes was conducted using media and environmental means in comparison to the industrial glycolipid producer, Candida bombicola. Proteomic profiles of yeast cells grown with or without palm oil uncovered novel key metabolic enzymes, namely fatty acid biosynthetic enzymes, leading to formation of glycolipid precursors. qRT-PCR identified some cluster genes responsible for biosynthesis of desirable glycolipids. Finally, LC-MS-based lipidomics of glycolipid fraction identified 15-(2'-O-β-D-glucopyranosyl-β-D-glucopyranosyloxy)hexadecanoic acid 1',4″-lactone 6',6″-diacetate (663.4525 m/z) as a major product. Using co-carbon substrates in the presence of salt and zinc, maximum glycolipid yield was achieved (55.72 g/L) with 55.30% emulsification activity and 10 mg/L of CMCs. Mixed glycolipids demonstrated antibiofilm activity against Candida albicans shown by reduction of metabolic activity. The novel biosurfactant-producing yeast M. guilliermondii MX is a promising cell factory of new antibiofilm glycolipids with potential for industrial-scale up.
生物表面活性剂比合成表面活性剂具有诸多优势，包括生物可降解性、环境友好性和低毒性。本研究采用季也蒙毕赤酵母MX菌株将木质纤维素木糖和棕榈油生物转化为具有理想特性的有价值的糖脂生物表面活性剂。目的是阐明与糖脂生产及其功能特性相关的代谢途径。为提高从头合成糖脂的产量，与工业糖脂生产者解脂假丝酵母相比，使用培养基和环境手段对相关酶基因进行了调控。在有或没有棕榈油的情况下培养的酵母细胞的蛋白质组学图谱揭示了新的关键代谢酶，即脂肪酸生物合成酶，其导致糖脂前体的形成。定量逆转录聚合酶链反应鉴定了一些负责合成所需糖脂的簇基因。最后，基于液相色谱-质谱联用的糖脂组分脂质组学鉴定出15-(2'-O-β-D-吡喃葡萄糖基-β-D-吡喃葡萄糖氧基)十六烷酸1',4″-内酯6',6″-二乙酸酯(质荷比为663.4525)为主要产物。在盐和锌存在的情况下使用共碳底物，实现了最大糖脂产量(55.72 g/L)，乳化活性为55.30%，临界胶束浓度为10 mg/L。混合糖脂对白色念珠菌表现出抗生物膜活性，表现为代谢活性降低。新型产生物表面活性剂酵母季也蒙毕赤酵母MX是一种有前景的细胞工厂，可生产具有工业规模放大潜力的新型抗生物膜糖脂。
