Kiran George Seghal, Sabarathnam Balu, Selvin Joseph
Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India.
FEMS Immunol Med Microbiol. 2010 Aug;59(3):432-8. doi: 10.1111/j.1574-695X.2010.00698.x. Epub 2010 May 12.
The antibiofilm activity of a glycolipid biosurfactant isolated from the marine actinobacterium Brevibacterium casei MSA19 was evaluated against pathogenic biofilms in vitro. The isolate B. casei MSA19 was a potential biosurfactant producer among the 57 stable strains isolated from the marine sponge Dendrilla nigra. The biosurfactant production was optimized under submerged fermentation. The purified glycolipid showed a broad spectrum of antimicrobial activity. Based on the minimum inhibitory concentration/minimum bactericidal concentration ratio, the glycolipid was determined as bacteriostatic. The glycolipid biosurfactant disrupted the biofilm formation under dynamic conditions. The disruption of the biofilm by the MSA19 glycolipid was consistent against mixed pathogenic biofilm bacteria. Therefore, the glycolipid biosurfactant can be used as a lead compound for the development of novel antibiofilm agents.
评估了从海洋放线菌酪短杆菌MSA19中分离出的一种糖脂生物表面活性剂对致病性生物膜的体外抗生物膜活性。酪短杆菌MSA19分离株是从海洋海绵黑指软珊瑚中分离出的57株稳定菌株中一种潜在的生物表面活性剂产生菌。在深层发酵条件下对生物表面活性剂的产量进行了优化。纯化后的糖脂表现出广谱抗菌活性。根据最低抑菌浓度/最低杀菌浓度比值，该糖脂被确定为抑菌剂。该糖脂生物表面活性剂在动态条件下破坏生物膜的形成。MSA19糖脂对混合致病性生物膜细菌的生物膜破坏作用是一致的。因此，该糖脂生物表面活性剂可作为开发新型抗生物膜药物的先导化合物。
