Puerarin Inhibits the Development of Thyroid Cancer Through KLF2/NOTCH1 Signaling.

BACKGROUND

Puerarin is the major bioactive ingredient extracted from Pueraria lobata. Puerarin has an antitumor effect on many kinds of cancer. Accordingly, the aim of this study was to investigate the effect and mechanism of puerarin on thyroid cancer (TC) proliferation and apoptosis.

METHODS

TC and normal thyroid cells experienced exposure to puerarin at 0, 10, 50, or 100 μg/mL. Impacts of short hairpin RNA of Kruppel-like factor 2 (sh), overexpression, and notch receptor 1 () overexpression on the malignant biological phenotypes of TC cells were gauged by cell function experiments. Quantification of KLF2, NOTCH1, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X (Bax), and Cleaved caspase 3 was completed using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analyses. and levels in TC were analyzed using the Encyclopedia of RNA Interactomes (ENCORI) project database. Through rescue experiments, whether the anti-tumor effect of puerarin on TC is realized via / axis was dissected.

RESULTS

Puerarin inhibited the malignant growth of TC cells by up-regulating , which was reversed by sh. was lowly expressed in TC. Notably, was negatively regulated by and was highly expressed in TC. overexpression abrogated overexpression-inhibited malignant growth of TC cells, which was manifested as increased cell proliferation and Bcl-2 as well as decreased cell apoptosis, Bax and Cleaved caspase 3.

CONCLUSION

Puerarin suppresses TC cell proliferation and apoptosis via the / axis.