Guo Zhuolin, Zhang Zhiheng, Guo Xindeng, Yang Weiwei, Liang Zhiqing, Ou Jinying, Cao Huihui, Lu Zibin, Yu Linzhong, Liu Junshan
Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, Guangdong Basic Research Center of Excellence for Integrated Traditional and Western Medicine for Qingzhi Diseases, Guangdong Provincial Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases,School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2025 May 20;45(5):1003-1012. doi: 10.12122/j.issn.1673-4254.2025.05.13.
To compare the anti-inflammatory, antibacterial and analgesic effects of Powder (YQS) formulated granules and decoction.
We first evaluated the anti-inflammatory effects of the two dosage forms of YQS in a LPS-induced RAW 264.7 cell model using RT-qPCR and Western blotting. We further constructed zebrafish models of inflammation by copper sulfate exposure, caudal fin transection, or LPS and Poly (I:C) microinjection, and evaluated anti-inflammatory effects of YQS granules and decoction by examining neutrophil aggregation and HE staining findings. In a mouse model of acute lung injury (ALI) induced by intratracheal LPS instillation, the effects of YQS gavage at 10, 15, and 20 g/kg on lung pathologies were evaluated by calculating lung wet-dry weight ratio and using HE staining, ELISA and Western blotting. The microbroth dilution method was used to evaluate the antibacterial effect of YQS. Mouse pain models established by hot plate and intraperitoneal injection of glacial acetic acid were used to evaluate the analgesic effects of YQS at 10, 15, and 20 g/kg.
Both YQS granules and decoction significantly reduced TNF-α, IL-6, and IL-1β expressions and p-STAT3 (Tyr 705) phosphorylation level in LPS-induced RAW 264.7 cells, and obviously inhibited neutrophil aggregation in the zebrafish models. In ALI mice, YQS granules and decoction effectively ameliorated lung injury, lowered lung wet-dry weight ratio, and reduced p-STAT3 (Tyr 705) expression and TNF-α and IL-6 levels. YQS produced obvious antibacterial effect at the doses of 15.63 and 31.25 mg/mL, and significantly reduced body torsion and increased pain threshold in the mouse pain models.
The two dosage forms of TQS have similar anti-inflammatory, antibacterial and analgesic effects with only differences in their inhibitory effect on TNF-α, IL-6 and IL-1β mRNA expressions in LPS-induced RAW 264.7 cells.
比较益气升陷汤（YQS）配方颗粒与汤剂的抗炎、抗菌及镇痛作用。
我们首先在脂多糖（LPS）诱导的RAW 264.7细胞模型中，使用逆转录定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹法（Western blotting）评估YQS两种剂型的抗炎作用。我们通过硫酸铜暴露、尾鳍横切或LPS与聚肌苷酸:聚胞苷酸（Poly (I:C)）显微注射构建斑马鱼炎症模型，并通过检测中性粒细胞聚集和苏木精-伊红（HE）染色结果评估YQS颗粒和汤剂的抗炎作用。在气管内滴注LPS诱导的急性肺损伤（ALI）小鼠模型中，通过计算肺湿重与干重比值，并使用HE染色、酶联免疫吸附测定（ELISA）和蛋白质免疫印迹法评估10、15和20 g/kg剂量的YQS灌胃对肺部病理的影响。采用微量肉汤稀释法评估YQS的抗菌作用。使用热板法和腹腔注射冰醋酸建立的小鼠疼痛模型，评估10、15和20 g/kg剂量的YQS的镇痛作用。
YQS颗粒和汤剂均显著降低LPS诱导的RAW 264.7细胞中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的表达以及磷酸化信号转导子和转录激活子3（p-STAT3，Tyr 705）水平，并明显抑制斑马鱼模型中的中性粒细胞聚集。在ALI小鼠中，YQS颗粒和汤剂有效改善肺损伤，降低肺湿重与干重比值，并降低p-STAT3（Tyr 705）表达以及TNF-α和IL-6水平。YQS在15.63和31.25 mg/mL剂量下产生明显的抗菌作用，并显著减少小鼠疼痛模型中的身体扭转次数并提高痛阈。
YQS的两种剂型具有相似的抗炎、抗菌和镇痛作用，仅在对LPS诱导的RAW 264.7细胞中TNF-α、IL-6和IL-1β mRNA表达的抑制作用上存在差异。
