Yamashita Tatsuya, Pires-daSilva Andre, Oomura Shun, Kusano Taichi, Haruta Nami, Hasumi Mayu, Kikuchi Taisei, Adams Sally, Sugimoto Asako, Shinya Ryoji
School of Agriculture, Meiji University, Kanagawa, Japan.
School of Life Sciences, University of Warwick, Gibbet Hill, England, United Kingdom.
MicroPubl Biol. 2025 May 8;2025. doi: 10.17912/micropub.biology.001585. eCollection 2025.
Functional gene analysis tools in are often ineffective in other nematodes due to differences in gonadal morphology and transgene silencing. Here, we established a method to generate stable transgenic lines in the nematodes and using microparticle bombardment coupled with hygromycin B selection. Despite using non-codon-optimized GFP, transgenic strains expressing fluorescent markers were obtained in both species. Additionally, an codon-optimized RFP construct showed robust expression in all tissues. This method will be valuable for future studies into the unusual sex determination, viviparity, and stress resistance in and .
由于性腺形态和转基因沉默的差异，[文中提及的某种线虫中]的功能基因分析工具在其他线虫中往往无效。在此，我们建立了一种方法，通过微粒轰击结合潮霉素B筛选，在[两种线虫名称]线虫中产生稳定的转基因品系。尽管使用的是未进行密码子优化的绿色荧光蛋白（GFP），但在这两个物种中均获得了表达荧光标记的转基因菌株。此外，一种[某种线虫名称]密码子优化的红色荧光蛋白（RFP）构建体在所有组织中均表现出强劲表达。该方法对于未来研究[两种线虫名称]线虫中异常的性别决定、胎生和抗逆性将具有重要价值。
