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某分类的系统发育以及绿色合成的氧化锌纳米颗粒对赭曲霉毒素的潜在抑制作用

Phylogeny of section and inhibition of ochratoxins potential by green synthesised ZnO nanoparticles.

作者信息

Hussein Mohamed A, Gherbawy Youssuf A, Abd El-Sadek Mahmoud S, F Al-Harthi Helal, Gam El-Dawy Eman

机构信息

Botany and Microbiology Department, Faculty of Science, South Valley University, Qena, Egypt.

Applied and Environmental Microbiology Center, South Valley University, Qena, Egypt.

出版信息

Mycology. 2024 Aug 27;16(2):891-902. doi: 10.1080/21501203.2024.2379480. eCollection 2025.

DOI:10.1080/21501203.2024.2379480
PMID:40415920
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12096659/
Abstract

Contamination of agricultural and industrial products by section becomes a true problem, especially, because a lot of species in this section can yield ochratoxins and other toxins. In this study, morphological criteria and partial calmodulin gene were used to identify 34 strains belonging to section isolated from and plants. The population is characterised by . , . , . , and . . The polyketide synthase () gene involved in ochratoxins (OTA) production was investigated by 1, 2 and 35-12L, R primers. Fifteen strains belonging to . were positive for gene, whilst . , . , and . were negative. All tested strains were able to produce ochratoxin A with different levels of 0.020-53 ppm except one isolate of . (IAEMAo6). The green synthesised ZnO-NPs have a significant inhibitory effect on OTA production by and . .

摘要

该菌属对农产品和工业产品的污染成为一个切实存在的问题，特别是因为该菌属中的许多物种能够产生赭曲霉毒素和其他毒素。在本研究中，利用形态学标准和部分钙调蛋白基因对从[具体植物1]和[具体植物2]植物中分离出的属于该菌属的34个菌株进行了鉴定。该菌群的特征为[具体特征1]、[具体特征2]、[具体特征3]、[具体特征4]和[具体特征5]。采用1、2和35 - 12L、R引物对参与赭曲霉毒素（OTA）产生的聚酮合酶（PKS）基因进行了研究。属于[具体菌属1]的15个菌株PKS基因呈阳性，而[具体菌属2]、[具体菌属3]、[具体菌属4]和[具体菌属5]为阴性。除[具体菌株名称]（IAEMAo6）的一个分离株外，所有测试菌株均能够产生水平为0.020 - 53 ppm的不同水平的赭曲霉毒素A。绿色合成的ZnO - NPs对[具体菌属1]和[具体菌属2]产生OTA具有显著的抑制作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/e9daa4cddbcd/TMYC_A_2379480_F0006_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/83f0b6ff9bc4/TMYC_A_2379480_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/c66049b7906c/TMYC_A_2379480_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/466ad46af615/TMYC_A_2379480_F0003_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/6d351bb8dab1/TMYC_A_2379480_F0004_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/9527102c933b/TMYC_A_2379480_F0005_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/e9daa4cddbcd/TMYC_A_2379480_F0006_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/83f0b6ff9bc4/TMYC_A_2379480_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/c66049b7906c/TMYC_A_2379480_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/466ad46af615/TMYC_A_2379480_F0003_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/6d351bb8dab1/TMYC_A_2379480_F0004_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/9527102c933b/TMYC_A_2379480_F0005_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e8/12096659/e9daa4cddbcd/TMYC_A_2379480_F0006_B.jpg

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