Nikiforova N V, Perepechkina N P, Varshavskiĭ V A, Pal'tsev M A, Shaldaeva V V
Biull Eksp Biol Med. 1985 Sep;100(9):377-80.
An active nephrotoxic serum (NTS) was prepared by immunization of rabbits with isolated renal glomeruli of rats and Freund's complete adjuvant. The glomeruli were isolated from the renal cortex by the sieving procedure. Intravenous injection of the NTS to rats induced the development of marked proteinuria and the nephrotic syndrome. Histological and electron microscopic studies of renal tissue performed by days 5 and 15 since the first injection of the NTS revealed extracapillary proliferative glomerulonephritis. IgG depositions on the basal membranes of the glomerular capillaries were linear on day 5 and granular on day 15 since the first injection of the NTS.
通过用大鼠分离的肾小球和弗氏完全佐剂免疫兔子制备了活性肾毒性血清（NTS）。通过筛分程序从肾皮质分离肾小球。给大鼠静脉注射NTS可诱发明显的蛋白尿和肾病综合征。自首次注射NTS起第5天和第15天对肾组织进行的组织学和电子显微镜研究显示为毛细血管外增生性肾小球肾炎。自首次注射NTS起第5天，肾小球毛细血管基底膜上的IgG沉积呈线性，第15天呈颗粒状。
