Pilot Study: Lymphatic Brain Decongestion: A New Surgical Technique in Alzheimer Disease.

BACKGROUND

Alzheimer disease (AD) is characterized by progressive cognitive function decline and neurodegeneration. Accumulation of amyloid-beta peptides forms plaques leading to brain inflammation and neuronal death. Glymphatic and meningeal lymphatic systems regulate brain fluid homeostasis and affect neurodegeneration by transferring amyloid-beta peptides from the brain to the cervical lymph nodes. We aimed to assess the feasibility of a novel operation in a rat model, to enhance the meningeal lymphatic system and consequently increase the clearance of amyloid-beta peptide.

METHODS

Ten 12-month-old rats were divided into 2 groups. Group A (control) had no intervention. Group B underwent collagen scaffold implantation between the subdural space above the hippocampus and the right submandibular lymph node, to achieve guided lymphangiogenesis. Animals were weighed and examined for neurological, behavioral and cognitive tests preoperatively and 6 and 12 weeks postoperatively, including NeuroScale, modified open field, and novel object location recognition tests.

RESULTS

One animal of group B died 2 days postoperatively. The remaining rats had no complications, maintained or increased their weight, and showed no signs of infection or neurological impairment. Behavioral tests were in favor of group B at 12 weeks postoperative. Animals of group A had statistically significant decline in cognitive function over time. Animals of group B demonstrated the same or better cognitive function.

CONCLUSIONS

In this pilot study, we demonstrated the feasibility of the proposed surgical procedure, which resulted in positive cognitive function for the animals. We are now utilizing an AD rat model to evaluate the possible therapeutic effect of the procedure.