Jin Peiyuan, Jiang Shan, Qiao Panpan, Dong Yinglu, Zhou Yuxin, Jung Geunhwa, Yang Zhimin, Hu Jian
College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, China.
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.
Pest Manag Sci. 2025 Sep;81(9):5750-5762. doi: 10.1002/ps.8928. Epub 2025 May 26.
Clarireedia spp. are a group of highly destructive phytopathogenic fungi that affect both cool- and warm-season turfgrasses, leading to the development of dollar spot (DS) disease. Biological control presents a promising alternative to chemical methods, offering environmental benefits and eliminating the risk of fungicide resistance development.
A total of 2934 endophytic bacterial strains isolated from turfgrasses were screened for their potential to suppress DS disease in this study. Forty-three strains demonstrated inhibitory effects against Clarireedia jacksonii isolate LT586, with growth inhibition rates (GIRs) ranging from 6.52% to 87.38%. Twenty-eight strains (primarily Pseudomonas and Bacillus spp.) showed > 50% GIR, and 11 of these strains effectively suppressed LT586 without causing turfgrass phytotoxicity. Pot experiments further validated the biocontrol potential of Bacillus cereus strain B694, which exhibited the highest control efficacy, with 92.93% inhibition at 6 days and 88.05% at 11 days post-inoculation. Scanning electron microscopy revealed that B694 formed biofilms on hyphae, degrading its cell walls and inducing atrophy. Transcriptome analysis of B694-treated C. jacksonii LT586 identified 1276 differentially expressed genes (1023 up-regulated; 253 down-regulated). Representative up-regulated genes were associated with chitin and glucan synthesis, sterol and fatty acid metabolism, glutathione metabolism, transmembrane transporters, and cytochrome P450 monooxygenases. These findings suggest that C. jacksonii attempts to maintain cellular function by enhancing cell wall and membrane synthesis, activating antioxidant stress responses, and detoxifying bacterial antimicrobial compounds.
This study provides a theoretical foundation for future investigations into the use of Bacillus cereus B694 as a potential biocontrol agent for DS in turfgrass disease management. © 2025 Society of Chemical Industry.
克拉里菌属是一类极具破坏性的植物病原真菌，会影响冷季型和暖季型草坪草，导致币斑病（DS）的发生。生物防治是化学方法的一种有前景的替代方案，具有环境效益并消除了产生抗真菌剂抗性的风险。
本研究从草坪草中分离出2934株内生细菌菌株，筛选它们抑制DS病的潜力。43株菌株对克拉里菌属的杰克逊氏菌分离株LT586表现出抑制作用，生长抑制率（GIRs）在6.52%至87.38%之间。28株菌株（主要是假单胞菌属和芽孢杆菌属）的GIR>50%，其中11株菌株有效抑制LT586且未对草坪草造成植物毒性。盆栽试验进一步验证了蜡样芽孢杆菌菌株B694的生物防治潜力，其在接种后6天的防治效果最高，抑制率为92.93%，11天时为88.05%。扫描电子显微镜显示，B694在菌丝上形成生物膜，降解其细胞壁并诱导萎缩。对经B694处理的杰克逊氏菌LT586进行转录组分析，鉴定出1276个差异表达基因（1023个上调；253个下调）。代表性的上调基因与几丁质和葡聚糖合成、甾醇和脂肪酸代谢、谷胱甘肽代谢、跨膜转运蛋白以及细胞色素P450单加氧酶有关。这些发现表明，杰克逊氏菌试图通过增强细胞壁和膜的合成、激活抗氧化应激反应以及对细菌抗菌化合物进行解毒来维持细胞功能。
本研究为未来将蜡样芽孢杆菌B694用作草坪草病害管理中DS的潜在生物防治剂的研究提供了理论基础。©2025化学工业协会。
