Jankowska E
Brain Res. 1985 Aug 26;341(2):403-8. doi: 10.1016/0006-8993(85)91084-4.
Factors affecting the retrograde transneuronal transport of wheat germ agglutinin conjugated with horseradish peroxidase (WGA-HRP), from spinal motoneurones to interneurones, have been studied in the cat. To this end, the location of transneuronally labelled interneurones was compared in animals which were awake or remained anaesthetized after WGA-HRP had been injected into the semitendinosus or the medial gastrocnemius nerve. In the anaesthetized animals motor axons of the injected nerves were stimulated selectively, to activate only Renshaw cells, or together with group I afferents, to activate also laminae V-VI interneurones with input from these muscles. The interneurones labelled in this study were distributed in different spinal cord regions than the interneurones labelled in preparations in which group I afferents of antagonist muscles were stimulated, as described in a previous study. The reported observations extend evidence of Harrison et al. that the retrograde transneuronal transport of WGA-HRP is facilitated by synaptic activity.
在猫身上研究了影响与辣根过氧化物酶结合的小麦胚凝集素（WGA-HRP）从脊髓运动神经元向中间神经元逆行跨神经元运输的因素。为此，将WGA-HRP注入半腱肌或内侧腓肠肌神经后，比较了清醒或仍处于麻醉状态的动物中经跨神经元标记的中间神经元的位置。在麻醉动物中，选择性刺激注入神经的运动轴突，仅激活闰绍细胞，或与I类传入纤维一起刺激，以激活也接受这些肌肉输入的V-VI层中间神经元。与先前研究中描述的刺激拮抗肌I类传入纤维的标本中标记的中间神经元相比，本研究中标记的中间神经元分布在不同的脊髓区域。所报告的观察结果扩展了哈里森等人的证据，即突触活动促进WGA-HRP的逆行跨神经元运输。
