Constant Field Gel Electrophoresis Analysis for DNA Double-Strand Break and Its Repair.

Constant field gel electrophoresis (CFGE) is the assay used to measure DNA strand breaks from the analysis of DNA fragment mobility through gel matrices based on their charge and size as pulsed field gel electrophoresis (PFGE). CFGE applies a continuous electric field to separate DNA fragments, while PFGE employs an alternating electric field to detect larger DNA fragments. Despite PFGE advancements, CFGE remains essential for its simplicity, cost-effectiveness, and efficacy to detect DNA fragments. It contributes globally to visualizing DNA damage and understanding cellular responses for genomic stability research.