Yu Jing, Ding Yanzi, Zhang Xue, Fang Yang, Tai Shuhong, Yuan Enwu, Duan Yitao
Department of Laboratory Medicine, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, People's Republic of China; Zhengzhou Key Laboratory for In Vitro Diagnosis of Hypertensive Disorders of Pregnancy, Zhengzhou, Henan, People's Republic of China.
Department of Laboratory Medicine, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, People's Republic of China; Zhengzhou Key Laboratory for In Vitro Diagnosis of Hypertensive Disorders of Pregnancy, Zhengzhou, Henan, People's Republic of China.
J Glob Antimicrob Resist. 2025 May 24;44:15-20. doi: 10.1016/j.jgar.2025.05.019.
To study the molecular characteristics, antibiotic resistance profiles and virulence features of Staphylococcus aureus (SA) isolates that cause mastitis.
117 SA isolates were collected from women with mastitis. Strain identification and antimicrobial susceptibility testing were conducted using the Vitek 2 system. All SA isolates were sequenced on the Illumina HiSeq platform. Multilocus sequence typing (MLST), ResFinder, BLAST against virulence factors, spaTyper, and SCCmecFinder were employed to analyse the strains.
35 methicillin-resistant Staphylococcus aureus (MRSA) strains and 82 methicillin-sensitive Staphylococcus aureus (MSSA) strains were isolated from women with acute breast abscesses. The rates of resistance to various antibiotics were significantly higher among MRSA isolates than among MSSA isolates. 22 sequence types (STs), 35 staphylococcal protein A (spa) types, and 4 SCCmec types were identified. ST22, ST59, and ST398 were the major lineages, and t309 and t437 were the most common spa types. SCCmec-IVa was the predominant SCCmec type. Interestingly, toxin gene subtypes A (hlgA, hlgB, hlgC, lukF-PV, lukS-PV, seg, sei, sem, sen, seo, seu, n = 54), B (hlgA, hlgB, hlgC, seb, sek, seq, n = 13), C (hlgA, hlgB, hlgC, lukD, lukE, n = 10), and D (hlgA, hlgB, hlgC, n = 10) accounted for 74.4% (87/117) of all SA isolates, suggesting the high expression of virulence genes.
ST22, ST398, and ST59 are the main types that cause mastitis and have different virulence factor profiles. This study provides deeper insights into the molecular epidemiology of SA associated with acute mastitis.
研究引起乳腺炎的金黄色葡萄球菌（SA）分离株的分子特征、抗生素耐药谱及毒力特征。
从乳腺炎患者中收集117株SA分离株。使用Vitek 2系统进行菌株鉴定和抗菌药物敏感性试验。所有SA分离株均在Illumina HiSeq平台上进行测序。采用多位点序列分型（MLST）、ResFinder、针对毒力因子的BLAST、spa分型及SCCmec分型对菌株进行分析。
从急性乳房脓肿患者中分离出35株耐甲氧西林金黄色葡萄球菌（MRSA）菌株和82株甲氧西林敏感金黄色葡萄球菌（MSSA）菌株。MRSA分离株对各种抗生素的耐药率显著高于MSSA分离株。鉴定出22种序列类型（STs）、35种葡萄球菌蛋白A（spa）类型和4种SCCmec类型。ST22、ST59和ST398是主要谱系，t309和t437是最常见的spa类型。SCCmec-IVa是主要的SCCmec类型。有趣的是，毒素基因亚型A（hlgA、hlgB、hlgC、lukF-PV、lukS-PV、seg、sei、sem、sen、seo、seu，n = 54）、B（hlgA、hlgB、hlgC、seb、sek、seq，n = 13）、C（hlgA、hlgB、hlgC、lukD、lukE，n = 10）和D（hlgA、hlgB、hlgC，n = 10）占所有SA分离株的74.4%（87/117），表明毒力基因高表达。
ST22、ST398和ST59是引起乳腺炎的主要类型，且具有不同的毒力因子谱。本研究为与急性乳腺炎相关的SA分子流行病学提供了更深入的见解。
