Jiao Wei, Zeng Nan, Hao Rui, Ma Hui, He Chao, He Honghui
Guangdong Research Center of Polarization Imaging and Measurement Engineering Technology, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK.
Biosensors (Basel). 2025 May 21;15(5):333. doi: 10.3390/bios15050333.
Mueller matrix polarimetry has great potential for tissue detection and clinical diagnosis due to its ability to provide rich microstructural information accurately. However, in practical in vivo tissue imaging based on backscattering Mueller matrix polarimetry, specular reflection is often inevitable, leading to overexposed regions and the following inaccurate polarization information acquisition of tissues. In this study, we probe the influence of specular reflection and overexposure on backscattering Mueller matrix polarimetry for tissue imaging and sensing. We investigate in detail the differentiation of polarization behaviors between the specular reflection and non-specular reflection tissue regions using a 3 × 3 backscattering Mueller matrix measurement. Then, we obtain the vertical projection profiles to further quantify the Mueller matrix elements of porcine liver tissue in different specular reflection regions. Finally, we calculate the polarization feature parameters derived from a 3 × 3 Mueller matrix and analyze their behavior in overexposed regions. Based on the quantitative analysis and comparisons, we obtain a group of polarization feature parameters with strong immunity to the specular reflection process. This study offers a strategy for selecting the polarization parameters during in vivo polarimetric imaging applications, provides valuable references for further eliminating the characterization errors induced by specular reflection, and may contribute to the advancement of quantitative tissue polarimetric imaging and sensing.
由于能够准确提供丰富的微观结构信息，穆勒矩阵偏振测量法在组织检测和临床诊断方面具有巨大潜力。然而，在基于背散射穆勒矩阵偏振测量法的实际体内组织成像中，镜面反射往往不可避免，导致出现过曝光区域以及随后组织偏振信息采集不准确的情况。在本研究中，我们探究了镜面反射和过曝光对用于组织成像与传感的背散射穆勒矩阵偏振测量法的影响。我们使用3×3背散射穆勒矩阵测量详细研究了镜面反射和非镜面反射组织区域之间偏振行为的差异。然后，我们获取垂直投影轮廓以进一步量化不同镜面反射区域中猪肝组织的穆勒矩阵元素。最后，我们计算从3×3穆勒矩阵导出的偏振特征参数，并分析它们在过曝光区域中的行为。基于定量分析和比较，我们获得了一组对镜面反射过程具有强抗性的偏振特征参数。本研究为体内偏振成像应用中的偏振参数选择提供了一种策略，为进一步消除由镜面反射引起的表征误差提供了有价值的参考，并可能有助于推进定量组织偏振成像与传感技术的发展。
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