Liu Teng, Sun Tao, He Honghui, Liu Shaoxiong, Dong Yang, Wu Jian, Ma Hui
Guangdong Research Center of Polarization Imaging and Measurement Engineering Technology, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
Department of Physics, Tsinghua University, Beijing 100084, China.
Biomed Opt Express. 2018 Aug 22;9(9):4413-4428. doi: 10.1364/BOE.9.004413. eCollection 2018 Sep 1.
Mueller matrix polarimetry is a potentially powerful tool for biomedical diagnosis. Recently, the transmission Mueller matrix microscope and backscattering Mueller matrix endoscope were developed and applied to various pathological samples. However, a comparative study of imaging contrasts of Mueller matrix derived parameters between transmission and backscattering measurements is still needed to help decide which information obtained from transmission Mueller matrix microscope can be directly applied to Mueller matrix imaging. Here, to compare the imaging contrasts of Mueller matrix derived parameters between transmission and backscattering polarimetry, we measure porcine liver tissue samples and human breast carcinoma tissue specimens. The experiments and corresponding Monte Carlo stimulation results demonstrate that the backscattering and transmission retardance-related Mueller matrix parameters have very similar contrasts to characterize the anisotropic and isotropic structures of pathological tissues, meaning that the conclusions made from Mueller matrix microscopic imaging based on retardance can also be helpful to guide the backscattering Mueller matrix polarimetric diagnosis. However, the values and contrasts of depolarization-related Mueller matrix parameters have some differences between transmission and backscattering polarimetry.
穆勒矩阵偏振测量法是一种用于生物医学诊断的潜在强大工具。最近，透射式穆勒矩阵显微镜和背散射穆勒矩阵内窥镜被开发出来并应用于各种病理样本。然而，仍需要对透射和背散射测量中穆勒矩阵衍生参数的成像对比度进行比较研究，以帮助确定从透射式穆勒矩阵显微镜获得的哪些信息可以直接应用于穆勒矩阵成像。在此，为了比较透射和背散射偏振测量中穆勒矩阵衍生参数的成像对比度，我们测量了猪肝组织样本和人乳腺癌组织标本。实验及相应的蒙特卡罗模拟结果表明，与背散射和透射延迟相关的穆勒矩阵参数在表征病理组织的各向异性和各向同性结构方面具有非常相似的对比度，这意味着基于延迟的穆勒矩阵显微成像得出的结论也有助于指导背散射穆勒矩阵偏振诊断。然而，与去偏振相关的穆勒矩阵参数的值和对比度在透射和背散射偏振测量之间存在一些差异。
