Xu Yunlong, Wang Jun, Tian Guangxiang, Liang Changcong, Zhou You, Guo Lijia, Yang Yang, Yang Laying
National Key Laboratory for Tropical Crop Breeding, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, National Collection of Microbial Resource for Fertilizer (Hainan), Collection of Tropical Agricultural Microbial Resource in Hainan Province, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
School of Plant Protection, Hainan University, Haikou 570228, China.
J Fungi (Basel). 2025 May 16;11(5):379. doi: 10.3390/jof11050379.
Banana wilt disease is an important disease in banana production, causing significant losses. Herein, we investigated the mechanism by which isolate X5 enhances the resistance of different resistant banana cultivars to f. sp. race 4 (Foc4). From the perspectives of metabolism, transcriptome, and key genes in important pathways, this study analyzed the composition and content changes of other types of signaling molecules, such as free amino acids and soluble sugars, in resistant/susceptible varieties. The results indicate that under pathogen stress, the contents of root-secreted metabolite components in both resistant and susceptible varieties increase significantly overall, and the increase in susceptible varieties is generally higher. For example, the free amino acid components in susceptible varieties are significantly more than those in resistant varieties. However, the addition of biocontrol bacteria can inhibit this increase. Exogenous addition experiments prove that differential metabolites can either promote or inhibit Foc4 and X5 at certain concentrations. The results of KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment and GO (Gene Ontology) annotation show that resistant varieties have more defense pathways compared to susceptible varieties. Under X5 treatment, more defense genes in resistant varieties are activated or their expression is enhanced, promoting the plant roots to secrete more substances related to plant resistance, such as phenylpropanoids and lignin. This research revealed the effects of on bananas and pathogens, allowing valuable conclusions to be drawn. The results have good application potential to understand the relationships among the three species, defining the biocontrol effect and mechanism of , and providing a theoretical basis for the biological control of soil-borne diseases such as banana wilt disease.
香蕉枯萎病是香蕉生产中的一种重要病害，会造成重大损失。在此，我们研究了分离株X5增强不同抗性香蕉品种对尖孢镰刀菌古巴专化型4号生理小种（Foc4）抗性的机制。本研究从代谢、转录组以及重要途径中的关键基因等角度，分析了抗性/感病品种中游离氨基酸和可溶性糖等其他类型信号分子的组成及含量变化。结果表明，在病原菌胁迫下，抗性和感病品种根系分泌的代谢物组分含量总体均显著增加，且感病品种的增加幅度通常更大。例如，感病品种中的游离氨基酸组分显著多于抗性品种。然而，添加生防菌可抑制这种增加。外源添加实验证明，差异代谢物在一定浓度下可促进或抑制Foc4和X5。京都基因与基因组百科全书（KEGG）富集和基因本体论（GO）注释结果表明，与感病品种相比，抗性品种具有更多的防御途径。在X5处理下，抗性品种中更多的防御基因被激活或表达增强，促使植物根系分泌更多与植物抗性相关的物质，如苯丙烷类化合物和木质素。本研究揭示了[具体研究对象]对香蕉和病原菌的影响，得出了有价值的结论。这些结果在理解三者之间的关系、明确[具体研究对象]的生防效果和机制以及为香蕉枯萎病等土传病害的生物防治提供理论依据方面具有良好的应用潜力。
