Horiguchi Takanori, Umeyama Takashi, Tomuro Hiroko, Otani Amato, Shinohara Takayuki, Abe Masahiro, Takatsuka Shogo, Miyazawa Ken, Nagi Minoru, Muraosa Yasunori, Hoshino Yasutaka, Sakoh Takashi, Araoka Hideki, Uchida Naoyuki, Kaneko Tomoyoshi, Nagano Yuko, Tsukada Hiroki, Miyazaki Taiga, Miyazaki Yoshitsugu
Department of Fungal Infection, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo 162-8640, Japan.
Division of Respirology, Rheumatology, Infectious Diseases and Neurology, Department of Internal Medicine, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
J Fungi (Basel). 2025 May 21;11(5):401. doi: 10.3390/jof11050401.
is a recently described yeast species related to and exclusively isolated from clinical specimens. However, its genomic features and pathogenic potential remain poorly understood. In this study, we performed whole-genome sequencing on three blood-derived isolates from patients with invasive fungal infections and comparative analyses with 13 related species. The three isolates yielded high-quality assemblies of 9-10 scaffolds (~21 Mb), facilitating reliable comparisons. While most species had comparable genome sizes, , , and displayed large, fragmented genomes, suggestive of polyploidy. ANI analysis and phylogenetic trees based on ANI and single-copy orthologs supported the classification of as a distinct clade with moderate intraspecific divergence. Using the , a model for fungal pathogenicity, all strains reduced larval survival, and NIIDF 0077300 exhibited virulence comparable to and greater than . To explore the gene-level differences associated with pathogenicity, we performed ortholog analysis based on single-copy genes. This revealed a unique Zn(II)Cys-type transcription factor gene (OG0010545) present only in NIIDF 0077300 and . These findings highlight the genomic diversity and infection-associated traits of , providing a framework for future functional studies.
是一种最近被描述的酵母物种，与……相关且仅从临床标本中分离得到。然而，其基因组特征和致病潜力仍知之甚少。在本研究中，我们对来自侵袭性真菌感染患者的三株血液分离株进行了全基因组测序，并与13个相关……物种进行了比较分析。这三株分离株产生了9 - 10个支架（约21 Mb）的高质量组装，便于进行可靠的比较。虽然大多数物种的基因组大小相当，但……、……和……显示出大的、碎片化的基因组，提示多倍体。基于ANI和单拷贝直系同源物的ANI分析和系统发育树支持将……分类为具有中等种内差异的独特进化枝。使用……，一种真菌致病性模型，所有……菌株均降低了幼虫存活率，且NIIDF 0077300表现出与……相当且大于……的毒力。为了探索与致病性相关的基因水平差异，我们基于单拷贝基因进行了直系同源物分析。这揭示了一个仅存在于NIIDF 0077300和……中的独特的Zn(II)Cys型转录因子基因（OG0010545）。这些发现突出了……的基因组多样性和与感染相关的特征，为未来的功能研究提供了框架。
