Naylor Trey R, Jacobs Mariana V, Elder Cameron J, Samaan Michael A, Clasey Jody L
Department of Kinesiology and Health Promotion, University of Kentucky, 216 Seaton Center, Lexington, KY 40502, USA.
Department of Pediatrics, University of Kentucky, Lexington, KY 40502, USA.
J Imaging. 2025 Apr 22;11(5):127. doi: 10.3390/jimaging11050127.
The purpose of this study was to determine the intra- and interrater reliability of quadriceps and hamstring soft tissue measures using DXA scans. A total of 44 subjects (23 males) participated in this study. The first total body DXA scan was performed in the standard anterior/posterior scanning position, followed by two additional total body scans while the subjects were lying on their left and right sides with the leg of interest extended and the contralateral leg bent. Unique regions of interest were created for analyses of mineral-free lean masses (MFL) using custom analysis software with manual tracing (by two investigators) of the quadriceps (QUADS) and hamstrings (HAMS) of the right and left thighs. Between-within repeated measure analysis of variance (ANOVA) was used to determine if there were significant differences among the MFL measures, while intraclass correlation coefficients (ICC) and coefficients of variation (CV) were used to assess the intra- and interrater reliability. Between-group analyses revealed that Investigator 2 had small yet significantly higher mean differences for right QUADS (2346.6 ± 602.4 g vs. 2327.4 ± 587.9 g), left QUADS (2337.3 ± 581.9 g vs. 2312.8 ± 581.2 g), right HAMS (2655.9 ± 626.3 g vs. 2543.0 ± 593.5 g), and left HAMS (2686.1 ± 628.1 g vs. 2562.8 ± 596.5 g) when compared to Investigator 1. Intraclass correlation coefficients between (≥0.984) and within (≥0.992) raters were high for all MFL measures, with low variation across all MFL measures (≤1.62%). Despite having significant group mean differences, our results revealed strong and significant reliability, and we recommend that a single investigator analyze the scans twice and that the mean of the two measures be used for final reporting within a given study.
本研究的目的是确定使用双能X线吸收法（DXA）扫描测量股四头肌和腘绳肌软组织的组内和组间可靠性。共有44名受试者（23名男性）参与了本研究。第一次全身DXA扫描在标准前后位扫描位置进行，随后进行另外两次全身扫描，受试者分别左侧卧和右侧卧，感兴趣的腿伸直，对侧腿弯曲。使用定制分析软件创建独特的感兴趣区域，通过手动追踪（由两名研究人员）左右大腿的股四头肌（QUADS）和腘绳肌（HAMS）来分析去矿物质瘦体重（MFL）。采用组间-组内重复测量方差分析（ANOVA）来确定MFL测量值之间是否存在显著差异，同时使用组内相关系数（ICC）和变异系数（CV）来评估组内和组间可靠性。组间分析显示，与研究人员1相比，研究人员2在右侧QUADS（2346.6±602.4 g对2327.4±587.9 g）、左侧QUADS（2337.3±581.9 g对2312.8±581.2 g）、右侧HAMS（2655.9±626.3 g对2543.0±593.5 g）和左侧HAMS（2686.1±628.1 g对2562.8±596.5 g）上的平均差异虽小但显著更高。所有MFL测量值的组间（≥0.984）和组内（≥0.992）评分者间相关系数都很高，所有MFL测量值的变异度都很低（≤1.62%）。尽管组间平均差异显著，但我们的结果显示出很强且显著的可靠性，我们建议在给定研究中由一名研究人员对扫描进行两次分析，并将两次测量的平均值用于最终报告。
