Gheorghe Catalina Gabriela, Dusescu-Vasile Cristina Maria, Popovici Daniela Roxana, Bombos Dorin, Dragomir Raluca Elena, Dima Floricel Maricel, Bajan Marian, Vasilievici Gabriel
Faculty of Petroleum Refining and Petrochemistry, Petroleum-Gas University of Ploiesti, 39 Bvd. Bucuresti, 100520 Ploiesti, Romania.
Institute for Research and Development in Aquatic Ecology, Fishing, and Aquaculture, 54 Portului Street, 800211 Galati, Romania.
Toxics. 2025 May 1;13(5):368. doi: 10.3390/toxics13050368.
The experiments in this study investigated the toxicity of naphthenic acids (NAs) on the algal culture . The tests monitored the progression of the algal suspension in media contaminated with various concentrations of naphthenic acids. The evolution of the algal culture during the metabolism of NAs was investigated. The monitoring also included the determination of the values of some parameters during the biodegradation process (pH, conductivity, cell viability, dissolved oxygen). Optical density measurements (OD) were used to quantify the growth of , alongside the determination of the sedimentation index (IS). Cell viability was assessed microscopically using TEM and optical microscopy. The results facilitated the estimation of the percentage of cell growth inhibition and the inhibitory concentration value, determined by estimating ECb50 (concentration of NAs corresponding to 50% inhibition). The chemical quantification of naphthenic acids in the samples analyzed was performed by calculating the acidity value (AV) and characterizing the naphthenic acids through FTIR analysis. The graphical representation of ECb50 was established by extrapolating to a concentration of 110 mg/mL of naphthenic acids. We have demonstrated that pollution caused by NAs can be mitigated by the algae , which can metabolize these compounds and thus biodegrade them.
本研究中的实验调查了环烷酸（NAs）对藻类培养物的毒性。测试监测了藻类悬浮液在被不同浓度环烷酸污染的培养基中的变化情况。研究了环烷酸代谢过程中藻类培养物的演变。监测还包括在生物降解过程中一些参数值的测定（pH值、电导率、细胞活力、溶解氧）。利用光密度测量（OD）来量化藻类的生长情况，同时测定沉降指数（IS）。使用透射电子显微镜（TEM）和光学显微镜通过显微镜观察来评估细胞活力。结果有助于估算细胞生长抑制百分比和抑制浓度值，通过估算ECb50（对应50%抑制的环烷酸浓度）来确定。通过计算酸度值（AV）并通过傅里叶变换红外光谱（FTIR）分析对环烷酸进行表征，对分析样品中的环烷酸进行化学定量。通过外推到110毫克/毫升的环烷酸浓度来建立ECb50的图形表示。我们已经证明，环烷酸造成的污染可以被藻类减轻，藻类可以代谢这些化合物并从而对其进行生物降解。
