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短期接触敌草隆后的毒性反应及游泳速度规律

Toxicity Response and Swimming Speed Regularity in After Short-Term Exposure to Diuron.

作者信息

Qin Feihu, Zhao Nanjing, Yin Gaofang, Luo Yunfei, Gan Tingting

机构信息

University of Science and Technology of China, Hefei 230026, China.

Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.

出版信息

Toxics. 2025 May 15;13(5):395. doi: 10.3390/toxics13050395.

Abstract

The agricultural production process contributes to the global issue of pesticide pollution. Based on the static toxicity test of diuron (DCMU) on () for EC-48 h, a concentration range of 0.2 to 1 mg/L was set as sublethal concentrations, while lethal concentrations were set at 2 mg/L and 4 mg/L. This study analyzes the toxic response patterns of the swimming behavior indicators of exposed to different concentrations of DCMU. The results showed that the average speed (V) of decreased step by step with exposure time, regardless of exposure to sublethal concentration or lethal concentration. However, during the same short-term exposure period, the V of at lethal concentration was higher than that at sublethal concentration, which indicates that the swimming behavior of exposed to DCMU may be stimulated and accelerated. Compared to the control group, there is a statistically significant difference in the V of after short-term exposure, especially showing an extremely significant difference after 5 min of exposure. Evidently, compared to the traditional 48 h static toxicity testing method, the swimming behavior indicators of show a more sensitive response to DCMU after 5 min of exposure, making it more suitable for rapid toxicity detection. By expanding the range of exposure concentrations, it was found that the V indicator of responded significantly to a DCMU concentration of 0.05 mg/L after only 5 min of exposure, and a high degree of correlation was observed between the indicator and the exposure concentration. Through nonlinear fitting, the relationship between V and the dose-effect of DCMU toxicity presents an S-shaped curve, with R > 0.9. Consequently, it becomes possible to study the dose-effect relationship between the changes in the swimming behavior indicators of and the stress concentration based on this theory. This further establishes a foundation for the development of comprehensive aquatic toxicity rapid detection technology based on the toxic response of swimming behavior indicators.

摘要

农业生产过程加剧了全球农药污染问题。基于敌草隆(DCMU)对()的48小时静态毒性试验,设定0.2至1毫克/升的浓度范围为亚致死浓度,而致死浓度设定为2毫克/升和4毫克/升。本研究分析了暴露于不同浓度DCMU的()游泳行为指标的毒性反应模式。结果表明,无论暴露于亚致死浓度还是致死浓度,()的平均速度(V)都随暴露时间逐步下降。然而,在相同的短期暴露期间,致死浓度下()的V高于亚致死浓度下的V,这表明暴露于DCMU的()的游泳行为可能会受到刺激并加速。与对照组相比,短期暴露后()的V存在统计学显著差异,尤其是在暴露5分钟后表现出极显著差异。显然,与传统的48小时静态毒性测试方法相比,暴露5分钟后()的游泳行为指标对DCMU表现出更敏感的反应,使其更适合快速毒性检测。通过扩大暴露浓度范围,发现仅暴露5分钟后,()的V指标对0.05毫克/升的DCMU浓度就有显著反应,并且该指标与暴露浓度之间存在高度相关性。通过非线性拟合,V与DCMU毒性的剂量效应关系呈现出S形曲线,R>0.9。因此,基于该理论研究()游泳行为指标变化与应激浓度之间的剂量效应关系成为可能。这进一步为基于游泳行为指标毒性反应的综合水生毒性快速检测技术的发展奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/12115561/403f21dc61c2/toxics-13-00395-g001.jpg

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