Kanemitsu F, Okigaki T
Clin Chim Acta. 1985 Sep 16;151(1):23-31. doi: 10.1016/0009-8981(85)90231-1.
Creatine kinase (ATP: creatine N-phosphotransferase, EC 2.7.3.2, CK) BB isoenzyme from stomach tumor tissue was partially purified and its characteristics were compared with those from healthy tissue. Molecular mass of tumor CK-BB was estimated to be 82 000 by polyacrylamide gel electrophoresis. Tumor CK-BB was separated into 2 main subbands around pH 4.5 and 11, minor subbands around pH 5-7.5 by agarose isoelectric focusing. The isoenzyme reacted with anti-human brain CK-BB antibodies and formed a hybrid, CK-MB, with CK-MM prepared from healthy human skeletal muscle. The above physicochemical and immunological characteristics of tumor CK-BB were the same as those of normal CK-BB from normal stomach tissue. Optimum pH of tumor CK-BB was more acidic than that of normal CK-BB. Affinity for creatine phosphate and heat sensitivity of tumor CK-BB were slightly lower than those of normal CK-BB. Tumor CK-BB was more stable after iodoacetamide and urea treatments.
从胃肿瘤组织中部分纯化了肌酸激酶（ATP：肌酸N-磷酸转移酶，EC 2.7.3.2，CK）BB同工酶，并将其特性与健康组织中的进行了比较。通过聚丙烯酰胺凝胶电泳估计肿瘤CK-BB的分子量为82000。通过琼脂糖等电聚焦，肿瘤CK-BB在pH 4.5和11左右分离为2个主要亚带，在pH 5-7.5左右为次要亚带。该同工酶与抗人脑CK-BB抗体反应，并与从健康人骨骼肌制备的CK-MM形成杂交体CK-MB。肿瘤CK-BB的上述物理化学和免疫学特性与正常胃组织中的正常CK-BB相同。肿瘤CK-BB的最适pH比正常CK-BB更偏酸性。肿瘤CK-BB对磷酸肌酸的亲和力和热敏感性略低于正常CK-BB。经碘乙酰胺和尿素处理后，肿瘤CK-BB更稳定。
