Jeong Eunwoo, Baek Youjin, Lee Hyeon Gyu
Department of Food and Nutrition, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea.
Department of Food and Nutrition, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea.
Food Chem. 2025 Oct 1;488:144809. doi: 10.1016/j.foodchem.2025.144809. Epub 2025 May 17.
Avenanthramide (AVN), a phenolamide unique to oats, offers health benefits but is limited in application due to poor solubility, instability, and low bioavailability. This study developed bilosomes (AOE-BL) by incorporating bile salts into liposomes (AOE-LP) as a cholesterol substitute to enhance the oral delivery of AVN-enriched oat extract (AOE). AOE-BL exhibited smaller particle sizes and higher encapsulation efficiency than AOE-LP. Both formulations significantly increased AVN solubility by 2.74-fold and 2.71-fold, respectively, compared to free AOE while preventing heat-induced degradation. AOE-BL demonstrated superior stability under acidic conditions, with less pronounced changes in particle size and zeta potential than AOE-LP. After in vitro digestion, AOE-BL achieved significantly higher bioaccessibility (77.31 ± 3.31 %) than AOE-LP (67.21 ± 2.37 %) and free AOE (55.69 ± 3.43 %). Cellular uptake studies in Caco-2 enterocytes revealed that bile salts enhanced intracellular uptake compared to cholesterol. These findings highlight the potential of AOE-BL as an effective oral delivery system for AVN.
燕麦酰胺（AVN）是燕麦特有的一种酚酰胺，具有健康益处，但由于溶解度差、稳定性低和生物利用度低，其应用受到限制。本研究通过将胆盐作为胆固醇替代物掺入脂质体（AOE-LP）中，开发了双分子层脂质体（AOE-BL），以增强富含AVN的燕麦提取物（AOE）的口服递送。与AOE-LP相比，AOE-BL表现出更小的粒径和更高的包封率。与游离AOE相比，两种制剂分别将AVN的溶解度显著提高了2.74倍和2.71倍，同时防止了热诱导的降解。AOE-BL在酸性条件下表现出优异的稳定性，与AOE-LP相比，粒径和zeta电位的变化较小。体外消化后，AOE-BL的生物可及性（77.31±3.31%）显著高于AOE-LP（67.21±2.37%）和游离AOE（55.69±3.43%）。在Caco-2肠细胞中的细胞摄取研究表明，与胆固醇相比，胆盐增强了细胞内摄取。这些发现突出了AOE-BL作为AVN有效口服递送系统的潜力。
