Emeriewen Ofere Francis, Wöhner Thomas Wolfgang, Flachowsky Henryk, Peil Andreas
ulius Kühn-Institut (JKI) - Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Dresden-Pillnitz, Germany.
Sci Data. 2025 May 27;12(1):873. doi: 10.1038/s41597-025-05232-0.
The wild apple, Malus fusca accession MAL0045, is highly resistant to fire blight disease, caused by the bacterial pathogen, Erwinia amylovora. A major resistance locus, FB_Mfu10 was identified on chromosome 10 of MAL0045 including other contributory loci on chromosomes 16, 4, and 15. Here, we report a chromosome-scale genome assembly of MAL0045 to facilitate the studies of its fire blight resistance. PacBio sequencing and Illumina sequencing for Hi-C contig anchorage were employed to obtain the genome. A total of 669.46 Mb sequences were anchored onto 17 chromosomes, taking up 99.75% of total contig length. Contigs anchored onto chromosomes were further ordered and orientated, where a total of 637.67 Mb sequences were anchored onto chromosomes in proper order and orientation, resulting in a final anchoring ratio of 95.25%. The BUSCO score of this assembly is 97.46%. Further, a total of 47,388 genes were predicted via ab initio, homology-based, and RNAseq methodologies. The availability of this genome will facilitate functional and comparative genomics studies, especially about the donors of fire blight resistance in Malus.
野生苹果品种Malus fusca的MAL0045 accession对由细菌病原体梨火疫欧文氏菌引起的火疫病具有高度抗性。在MAL0045的第10号染色体上鉴定出一个主要抗性位点FB_Mfu10，以及第16、4和15号染色体上的其他辅助位点。在此，我们报告了MAL0045的染色体水平基因组组装，以促进其对火疫病抗性的研究。采用PacBio测序和用于Hi-C重叠群锚定的Illumina测序来获得基因组。总共669.46 Mb的序列被锚定到17条染色体上，占重叠群总长度的99.75%。锚定到染色体上的重叠群进一步进行排序和定向，其中总共637.67 Mb的序列以正确的顺序和方向锚定到染色体上，最终锚定率为95.25%。该组装的BUSCO分数为97.46%。此外，通过从头预测、基于同源性和RNAseq方法总共预测了47,388个基因。这个基因组的可用性将促进功能和比较基因组学研究，特别是关于苹果属中火疫病抗性供体的研究。
