丝氨酸蛋白酶抑制剂A3（SERPINA3）在人类脂肪细胞中表达，并受肿瘤坏死因子-α（TNF-α）和维生素B6调节。
SERPINA3 is expressed in human adipocytes and modulated by TNF-α and vitamin B6.
作者信息
Guzmán-Herrera Nataly, Ruíz-Madrigal Bertha, Parés-Hipólito Jaime, Salazar-Olivo Luis A
机构信息
Molecular Biology Division, Instituto Potosino de Investigación Científica y Tecnológica, Camino a La Presa San José 2055, San Luis Potosí, 78216, México.
Microbiology Research Laboratory, Department of Microbiology and Pathology, University Center for Health Sciences, University of Guadalajara, Sierra Mojada 950, Guadalajara, 44340, Jalisco, México.
出版信息
In Vitro Cell Dev Biol Anim. 2025 May 27. doi: 10.1007/s11626-025-01053-y.
SERPINA3G participates in the antiadipogenesis and insulin resistance induced by TNF-α in 3T3-F442A murine cells. Here, we show that the human orthologue SERPINA3 is expressed in human subcutaneous and visceral adipose depots of normal-weight individuals and that TNF-α and RA induced the overexpression of SERPINA3 mRNA in cultured human subcutaneous and visceral adipocytes, although only TNF-α induced the expression of serpin A3 protein. We also demonstrate that vitamin B6 abrogated the expression of the SERPINA3 gene and diminished the anti-adipogenic effects of TNF-α on mature adipocytes. Our results indicate that SERPINA3 is expressed in human adipose tissues and modulates the antiadipogenic effects of TNF-α, and suggest serpin A3 could be a promissory target in the inflammatory processes linked to obesity and other adipose dysfunctions.
丝氨酸蛋白酶抑制剂A3G（SERPINA3G）参与肿瘤坏死因子-α（TNF-α）在3T3-F442A小鼠细胞中诱导的抗脂肪生成和胰岛素抵抗。在此，我们发现人类同源物丝氨酸蛋白酶抑制剂A3（SERPINA3）在正常体重个体的人类皮下和内脏脂肪库中表达，并且TNF-α和视黄酸（RA）可诱导培养的人类皮下和内脏脂肪细胞中SERPINA3 mRNA的过表达，尽管只有TNF-α能诱导丝氨酸蛋白酶抑制剂A3蛋白的表达。我们还证明，维生素B6可消除SERPINA3基因的表达，并减弱TNF-α对成熟脂肪细胞的抗脂肪生成作用。我们的结果表明，SERPINA3在人类脂肪组织中表达，并调节TNF-α的抗脂肪生成作用，提示丝氨酸蛋白酶抑制剂A3可能是与肥胖及其他脂肪功能障碍相关的炎症过程中的一个有前景的靶点。
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