黄鳍金枪鱼（Thunnus albacares）肌红蛋白还原酶的分离与鉴定。
Isolation and characterization of metmyoglobin reductase from yellowfin tuna (Thunnus albacares).
作者信息
Levy M J, Livingston D J, Criddle R S, Brown W D
出版信息
Comp Biochem Physiol B. 1985;81(4):809-14. doi: 10.1016/0305-0491(85)90070-7.
PMID:4042625
Abstract
The isolation, purification, and characterization of metmyoglobin reductase from yellowfin tuna (Thunnus albacares) is described. The enzyme has been purified 120-fold. Characterization of the enzyme includes molecular weight, isoelectric point, substrate specificity, enzyme kinetics, chromatographic behavior, and sensitivity to inhibitors. The physical and catalytic properties of the tuna enzyme are compared to those of bovine and blue-white dolphin metmyoglobin reductase.
摘要
本文描述了从黄鳍金枪鱼（Thunnus albacares）中分离、纯化和鉴定高铁肌红蛋白还原酶的过程。该酶已被纯化了120倍。对该酶的鉴定包括分子量、等电点、底物特异性、酶动力学、色谱行为以及对抑制剂的敏感性。将金枪鱼酶的物理和催化特性与牛和白暨豚高铁肌红蛋白还原酶的特性进行了比较。
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