Alvira-Arill Gustavo R, Herrera Oscar R, Stultz Jeremy S, Peters Brian M
Department of Clinical Pharmacy and Outcomes Sciences, College of Pharmacy, Medical University of South Carolina, Charleston, SC 29425, USA.
Department of Clinical Pharmacy and Translational Science, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Antibiotics (Basel). 2025 May 9;14(5):484. doi: 10.3390/antibiotics14050484.
: Compared to soybean oil intravenous fat emulsion (SO-IFE), use of mixed-oil IFE (MO-IFE) is associated with reduced rates of catheter-related bloodstream infections caused by coagulase-negative species (CoNS) in pediatric patients receiving parenteral nutrition. : Using an in vitro biofilm model, this study aimed to assess the impact of IFEs on biofilm formation among species. , , , , , and were cultivated as biofilms in media supplemented with SO-IFE, MO-IFE, or fish oil IFE (IFE). Biomass was quantified by the crystal violet method, and follow-up planktonic growth assays assessed antimicrobial effects of IFEs. : Compared to SO-IFE, MO-IFE and FO-IFE significantly inhibited biofilm formation of but did not impact planktonic growth. Contrary to clinical data, CoNS biofilm formation was not impacted by any of the IFEs tested. biofilm inhibition in IFEs was further investigated by comparing differences following growth in SO-IFE supplemented with capric acid, docosahexaenoic acid (DHA), or eicosapenaenoic acid (EPA) to concentrations matching those of MO-IFE. Capric acid supplementation was associated with significant reduction in biofilm formation compared to SO-IFE alone. However, this was attributed to a bactericidal effect based on follow-up planktonic growth assays. : These results suggest that biofilm formation in is variably impacted by fatty acid composition in clinically relevant IFEs, with capric acid exhibiting bactericidal activity against tested isolates.
与大豆油静脉脂肪乳剂（SO-IFE）相比，在接受肠外营养的儿科患者中，使用混合油脂肪乳剂（MO-IFE）可降低由凝固酶阴性菌（CoNS）引起的导管相关血流感染率。本研究旨在使用体外生物膜模型评估脂肪乳剂对不同菌种生物膜形成的影响。将表皮葡萄球菌、溶血葡萄球菌、头状葡萄球菌、人葡萄球菌、木糖葡萄球菌和模仿葡萄球菌在补充有SO-IFE、MO-IFE或鱼油脂肪乳剂（FO-IFE）的培养基中培养成生物膜。通过结晶紫法对生物量进行定量，并通过后续的浮游生长试验评估脂肪乳剂的抗菌效果。与SO-IFE相比，MO-IFE和FO-IFE显著抑制了表皮葡萄球菌的生物膜形成，但对浮游生长没有影响。与临床数据相反，所测试的任何一种脂肪乳剂均未影响CoNS的生物膜形成。通过比较在补充癸酸、二十二碳六烯酸（DHA）或二十碳五烯酸（EPA）的SO-IFE中生长至与MO-IFE浓度匹配后的差异，进一步研究了脂肪乳剂中对表皮葡萄球菌生物膜的抑制作用。与单独的SO-IFE相比，补充癸酸与生物膜形成的显著减少有关。然而，根据后续的浮游生长试验，这归因于杀菌作用。这些结果表明，临床相关脂肪乳剂中的脂肪酸组成对表皮葡萄球菌生物膜形成有不同影响，癸酸对测试菌株具有杀菌活性。
