中国猪繁殖与呼吸综合征1型非结构蛋白2基因的重组与遗传多样性分析
Recombination and Genetic Diversity Analysis of Porcine Reproductive and Respiratory Syndrome 1 Nonstructural Protein 2 Genes in China.
作者信息
Lv Chen, Guan Baoyi, Pang Jiankun, Kong Weili, Wang Ruining, Wang Lin, Zhao Mengmeng, Zhang Hang
机构信息
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, China.
Gladstone Institutes of Virology and Immunology, University of California, San Francisco, CA 94158, USA.
出版信息
Genes (Basel). 2025 Apr 28;16(5):507. doi: 10.3390/genes16050507.
BACKGROUND
Porcine reproductive and respiratory syndrome (PRRS) has been present in China for about 30 years, and because of the high mutability of PRRSV, it causes huge economic losses to pig enterprises every year. PRRSV-2 is widely prevalent in China, and the detection rate of PRRSV-1 is also on the rise. Nonstructural protein 2 (NSP2) is a highly variable protein with multiple biological functions, such as PRRSV replication, which plays an important role in understanding PRRSV variation and epidemic alerts.
OBJECTIVES
The epidemic characteristics and recombination of PRRSV-1 are still unknown. The purpose of this study is to study the epidemic characteristics of PRRSV-1 and lay a foundation for the prevention and control of PRRSV-1.
METHODS
In this study, we collected several PRRSV-1 and PRRSV-2 gene sequences for gene sequence and recombination analyses, aiming to analyze the recombination pattern and genetic variation in the PRRSV-1 genes in China.
RESULTS
The genetic similarity results showed that the 69 PRRSV-1 gene sequences collected in this study showed nucleotide similarity ranging from 67.3% to 100.0% and amino acid similarity ranging from 64.3% to 100.0%. Amino acid sequence comparison showed that PRRSV-1 had more amino acid deletion or substitution sites than PRRSV-2. NSP2 also contains special amino acid regions such as the highly immunogenic region. PRRSV-1 can be categorized into four strains, NMEU09-1-like, BJEU06-1-like, HKEU-16-like and Amervac-like isolates, and are at different positions in the ML and NJ phylogenetic trees. In the ninety selected PRRSVs, six recombination events were detected using recombination analysis, two of which occurred in Chinese PRRSV-1 strains. Therefore, sequence analysis of helps us to understand the prevalence and variation in PRRSV-1 in China over the past two decades and provides a theoretical basis for studying the epidemiology and evolution of .
背景
猪繁殖与呼吸综合征（PRRS）在中国已存在约30年，由于PRRSV的高变异性，每年给养猪企业造成巨大经济损失。PRRSV - 2在中国广泛流行，PRRSV - 1的检出率也在上升。非结构蛋白2（NSP2）是一种具有多种生物学功能的高度可变蛋白，如PRRSV复制，在理解PRRSV变异和疫情预警方面发挥着重要作用。
目的
PRRSV - 1的流行特征和重组情况仍不清楚。本研究的目的是研究PRRSV - 1的流行特征，为PRRSV - 1的防控奠定基础。
方法
在本研究中，我们收集了多个PRRSV - 1和PRRSV - 2基因序列进行基因序列和重组分析，旨在分析中国PRRSV - 1基因中的重组模式和遗传变异。
结果
遗传相似性结果表明，本研究收集的69个PRRSV - 1基因序列的核苷酸相似性范围为67.3%至100.0%，氨基酸相似性范围为64.3%至100.0%。氨基酸序列比较表明，PRRSV - 1比PRRSV - 2具有更多的氨基酸缺失或替换位点。NSP2还包含高免疫原性区域等特殊氨基酸区域。PRRSV - 1可分为四个毒株，即NMEU09 - 1样、BJEU06 - 1样、HKEU - 16样和Amervac样分离株，并且在ML和NJ系统发育树中的位置不同。在90个选定的PRRSV中，使用重组分析检测到6个重组事件，其中2个发生在中国PRRSV - 1毒株中。因此，序列分析有助于我们了解过去二十年中国PRRSV - 1的流行情况和变异情况，并为研究其流行病学和进化提供理论依据。
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