National Research Center for Veterinary Medicine, Luoyang, Cuiwei Road, High-Tech District, Luoyang, 471003, Henan Province, People's Republic of China.
National Research Center for Veterinary Medicine, Luoyang, Cuiwei Road, High-Tech District, Luoyang, 471003, Henan Province, People's Republic of China; WOAH Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, No. 17 Tiangui Road, Daxing District, Beijing, 100125, People's Republic of China.
Microb Pathog. 2023 Jul;180:106158. doi: 10.1016/j.micpath.2023.106158. Epub 2023 May 16.
PRRSV-1 has caused more clinical infections in pigs in Chinese swine herds in recent years, however, the pathogenicity of PRRSV-1 in China is unclear. In order to study the pathogenicity of PRRSV-1, in this study, a PRRSV-1 strain, 181187-2, was isolated in primary alveolar macrophage (PAM) cells from a farm where abortions had been reported in China. The complete genome of 181187-2 was 14932 bp excluding Poly A, with 54-amino acid continuous deletion in the Nsp2 gene and 1 amino deletion in ORF3 gene compared with LV. Additionally, the piglets inoculated with strain 181187-2 by intranasal and intranasal plus intramuscular injection, animal experiments showed clinical symptoms including transient fever and depression, with no death. The obvious histopathological lesions including interstitial pneumonia and lymph node hemorrhage, and there were no significant differences in clinical symptoms and histopathological lesions with different challenge ways. Our results indicated that PRRSV -1 181187-2 was a moderately pathogenic strain in piglets.
近年来，PRRSV-1 在中国猪群中引起了更多的临床感染，但 PRRSV-1 的致病性在中国尚不清楚。为了研究 PRRSV-1 的致病性，本研究从中国发生流产的农场中分离到一株 PRRSV-1 毒株 181187-2，该毒株在原代肺泡巨噬细胞（PAM）中培养。181187-2 的全基因组长度为 14932bp，不包括 Poly A，与 LV 相比，Nsp2 基因中有 54 个连续氨基酸缺失，ORF3 基因中有 1 个氨基酸缺失。此外，猪只通过鼻腔内和鼻腔内加肌肉内接种该毒株后，动物实验显示出短暂发热和抑郁等临床症状，没有死亡。明显的组织病理学病变包括间质性肺炎和淋巴结出血，不同接种方式的临床症状和组织病理学病变没有显著差异。我们的结果表明，PRRSV-1 181187-2 是一种中等毒力的猪源毒株。
