Harris L S, Gray G R
J Biol Chem. 1977 Apr 25;252(8):2470-7.
A polysaccharide composed of 3-O-methyl-D-mannose and D-mannose in a molar ratio of approximately 10:1 and containing 3 to 4 esterified acetyl residues has been isolated from Streptomyces griseus. This acetylated methylmannose polysaccharide (AMMP) is similar to the methylmannose polysaccharide (MMP) of Mycobacterium smegmatis (Gray, G. R., and Ballou, C. E. (1971) J. Biol. Chem. 246, 6835-6842) in its size and composition, the absence of acidic or basic groups, and the lack of a reducing end. It is different, however, in its content of esterified acetyl residues, and it is slightly different in its structure and in its gel filtration properties. The structure of AMMP has been established by proton magnetic resonance spectroscopy, and by combinations of methylation analysis and Smith degradation utilizing non-radioactively labeled polysaccharide and [3H]methyl-labeled polysaccharide obtained from cells grown in the presence of L-[methyl-3H]methionine. It is concluded that AMMP is a linear, nonreducing, neutral polysaccharide composed of a terminal D-mannose residue linked alpha(1 leads to 4) to a chain of 10 consecutive alpha(1 leads to 4)-linked 3-O-methyl-D-mannose residues. The reducing terminal 3-O-methyl-D-mannose residue exists, at least in part, as its alpha-methyl glycoside. The positions of attachment of the ester residues have not been established.
从灰色链霉菌中分离出了一种多糖，它由3-O-甲基-D-甘露糖和D-甘露糖以大约10:1的摩尔比组成，并含有3至4个酯化的乙酰基残基。这种乙酰化甲基甘露糖多糖（AMMP）在大小和组成、不存在酸性或碱性基团以及缺乏还原端方面，与耻垢分枝杆菌的甲基甘露糖多糖（MMP）（Gray, G. R., and Ballou, C. E. (1971) J. Biol. Chem. 246, 6835 - 6842）相似。然而，它在酯化乙酰基残基的含量上有所不同，在结构和凝胶过滤特性上也略有差异。AMMP的结构已通过质子磁共振光谱法，以及利用非放射性标记的多糖和从在L-[甲基-³H]甲硫氨酸存在下生长的细胞中获得的[³H]甲基标记的多糖进行甲基化分析和史密斯降解的组合来确定。得出的结论是，AMMP是一种线性、非还原、中性多糖，由一个末端D-甘露糖残基通过α(1→4)连接到由10个连续的α(1→4)连接的3-O-甲基-D-甘露糖残基组成的链上。还原末端的3-O-甲基-D-甘露糖残基至少部分以其α-甲基糖苷的形式存在。酯残基的连接位置尚未确定。
