Fouza Ariadni, Fylaktou Asimina, Daoudaki Maria, Talimtzi Persefoni, Tagkouta Anneta, Vagiotas Lampros, Katsanos Georgios, Tsoulfas Georgios, Antoniadis Nikolaos
Department of Transplant Surgery, Center for Research and Innovation in Solid Organ Transplantation, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Department of Immunology, National Peripheral Histocompatibility Center, Hippokration General Hospital of Thessaloniki, 54642 Thessaloniki, Greece.
J Clin Med. 2025 May 9;14(10):3312. doi: 10.3390/jcm14103312.
B lymphocytes are involved in both graft function and rejection. The role of double-negative (DN) and marginal zone B (MZB) lymphocytes in transplantation remains unclear. This study aims to investigate their role one year after transplant. The frequency and absolute numbers of DN and MZB cells were determined by flow cytometry before transplantation and at 3, 6 and 12 months after transplant. They were correlated with graft function and rejection. Both the frequency and absolute number of MZB and DN cells increased 12 months after transplantation. Variations were observed in the populations studied at different time points. The observed decrease in the frequency of MZB lymphocytes in kidney recipients with rejection at 12 months, the end of follow-up, was associated with rejection episodes. On ROC curve analysis, a cut-off value of <20.6% could be a predictor of rejection risk in the first 12 months after transplantation (sensitivity 72.7%, specificity 69.6%). No relationship was found between the frequencies and absolute numbers of cell populations and graft function at any time point. The kinetics of B cells (DN and MZB) were determined over the course of 12 months after kidney transplantation. The frequency of MZ B cells was associated with rejection episodes.
B淋巴细胞参与移植器官功能及排斥反应。双阴性（DN）和边缘区B（MZB）淋巴细胞在移植中的作用尚不清楚。本研究旨在探讨移植一年后它们的作用。通过流式细胞术在移植前以及移植后3个月、6个月和12个月测定DN和MZB细胞的频率和绝对数量。将它们与移植器官功能及排斥反应相关联。移植后12个月，MZB和DN细胞的频率和绝对数量均增加。在不同时间点研究的细胞群体中观察到了变化。在随访结束时即12个月时，出现排斥反应的肾移植受者中MZB淋巴细胞频率的下降与排斥反应发作相关。在ROC曲线分析中，<20.6%的临界值可能是移植后前12个月排斥风险的预测指标（敏感性72.7%，特异性69.6%）。在任何时间点，均未发现细胞群体的频率和绝对数量与移植器官功能之间存在关联。在肾移植后的12个月内确定了B细胞（DN和MZB）的动力学变化。MZ B细胞的频率与排斥反应发作相关。
